%0 Journal Article %1 RN977 %A Li, K. K. %A Prada, J. %A Damineli, D. S. C. %A Liese, A. %A Romeis, T. %A Dandekar, T. %A Feijó, J. A. %A Hedrich, R. %A Konrad, K. R. %D 2021 %J New Phytologist %K (aba) abscisic acid myOwn %N 6 %P 2292-2310 %R 10.1111/nph.17202 %T An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca and H reveals new insights into ion signaling in plants %U /brokenurl#<Go to ISI>://WOS:000619171500001 %V 230 %X Whereas the role of calcium ions (Ca2+) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca2+ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca2+- and pH-dynamics revealed interconnections previously not described. In tobacco PTs, we demonstrated Ca2+-dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca2+. In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca2+ elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca2+-increases and cytosolic acidification by c. 2 min, suggesting a Ca2+/pH-independent early pathogen signaling step. Imaging Ca2+ and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca2+- and pH-responses. We propose close interrelation in Ca2+- and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement.

@article{RN977, abstract = {Whereas the role of calcium ions (Ca2+) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca2+ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca2+- and pH-dynamics revealed interconnections previously not described. In tobacco PTs, we demonstrated Ca2+-dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca2+. In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca2+ elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca2+-increases and cytosolic acidification by c. 2 min, suggesting a Ca2+/pH-independent early pathogen signaling step. Imaging Ca2+ and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca2+- and pH-responses. We propose close interrelation in Ca2+- and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Li, K. K. and Prada, J. and Damineli, D. S. C. and Liese, A. and Romeis, T. and Dandekar, T. and Feijó, J. A. and Hedrich, R. and Konrad, K. R.}, biburl = {https://www.bibsonomy.org/bibtex/241a6b9dd5905b31cde7845eab5e23e8b/rainerhedrich_2}, doi = {10.1111/nph.17202}, interhash = {2eebbaefd97f620ea1c42d7d0ce4902f}, intrahash = {41a6b9dd5905b31cde7845eab5e23e8b}, issn = {0028-646x}, journal = {New Phytologist}, keywords = {(aba) abscisic acid myOwn}, note = {Sd1fk Times Cited:34 Cited References Count:147}, number = 6, pages = {2292-2310}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca and H reveals new insights into ion signaling in plants}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000619171500001}, volume = 230, year = 2021 }