%0 Journal Article %1 baudat2009prdm9 %A Baudat, F. %A Buard, J. %A Grey, C. %A Fledel-Alon, A. %A Ober, C. %A Przeworski, M. %A Coop, G. %A de Massy, B. %D 2009 %J Science %K PRDM9 drive hotspots %P science.1183439 %R 10.1126/science.1183439 %T PRDM9 Is a Major Determinant of Meiotic Recombination Hotspots in Humans and Mice %U http://www.sciencemag.org/cgi/content/abstract/science.1183439v1 %X Meiotic recombination events cluster into narrow segments of the genome, defined as hotspots. Here, we demonstrate that a major player for hotspot specification is the Prdm9 gene. First, two mouse strains that differ in hotspot usage are polymorphic for the zinc finger DNA binding array of PRDM9. Second, the human consensus PRDM9 allele is predicted to recognize the 13-mer motif enriched at human hotspots; this DNA binding specificity is verified by in vitro studies. Third, allelic variants of PRDM9 zinc fingers are significantly associated with variability in genome-wide hotspot usage among humans. Our results provide a molecular basis for the distribution of meiotic recombination in mammals, where the binding of PRDM9 to specific DNA sequences targets the initiation of recombination at specific locations in the genome.

@article{baudat2009prdm9, abstract = {Meiotic recombination events cluster into narrow segments of the genome, defined as hotspots. Here, we demonstrate that a major player for hotspot specification is the Prdm9 gene. First, two mouse strains that differ in hotspot usage are polymorphic for the zinc finger DNA binding array of PRDM9. Second, the human consensus PRDM9 allele is predicted to recognize the 13-mer motif enriched at human hotspots; this DNA binding specificity is verified by in vitro studies. Third, allelic variants of PRDM9 zinc fingers are significantly associated with variability in genome-wide hotspot usage among humans. Our results provide a molecular basis for the distribution of meiotic recombination in mammals, where the binding of PRDM9 to specific DNA sequences targets the initiation of recombination at specific locations in the genome. }, added-at = {2010-01-06T00:36:46.000+0100}, author = {Baudat, F. and Buard, J. and Grey, C. and Fledel-Alon, A. and Ober, C. and Przeworski, M. and Coop, G. and de Massy, B.}, biburl = {https://www.bibsonomy.org/bibtex/2f4806662b55e79fefbdd129b0f5e7b51/peter.ralph}, description = {PRDM9 Is a Major Determinant of Meiotic Recombination Hotspots in Humans and Mice -- Baudat et al., 10.1126/science.1183439 -- Science}, doi = {10.1126/science.1183439}, interhash = {74f4466cecb9e42484abc88f79472fab}, intrahash = {f4806662b55e79fefbdd129b0f5e7b51}, journal = {Science}, keywords = {PRDM9 drive hotspots}, pages = {science.1183439}, timestamp = {2010-01-06T00:36:46.000+0100}, title = {{PRDM9 Is a Major Determinant of Meiotic Recombination Hotspots in Humans and Mice}}, url = {http://www.sciencemag.org/cgi/content/abstract/science.1183439v1}, year = 2009 }