%0 Journal Article %1 SergeiNechaev12102009 %A Nechaev, Sergei %A Fargo, David C. %A dos Santos, Gilberto %A Liu, Liwen %A Gao, Yuan %A Adelman, Karen %D 2009 %J Science %K stalled_transcription transcription %P science.1181421 %R 10.1126/science.1181421 %T Global Analysis of Short RNAs Reveals Widespread Promoter-Proximal Stalling and Arrest of Pol II in Drosophila %U http://www.sciencemag.org/cgi/content/abstract/science.1181421v1 %X Emerging evidence indicates that gene expression in higher organisms is regulated by RNA polymerase II stalling during early transcription elongation. To probe the mechanisms responsible for this regulation, we developed methods to isolate and characterize short RNAs derived from stalled RNA polymerase II in Drosophila cells. Significant levels of these short RNAs were generated from over one-third of all genes, indicating that promoter-proximal stalling is a general feature of early polymerase elongation. Nucleotide composition of the initially transcribed sequence played an important role in promoting transcriptional stalling by rendering polymerase elongation complexes highly susceptible to backtracking and arrest. These results indicate that the intrinsic efficiency of early elongation can greatly affect gene expression.

@article{SergeiNechaev12102009, abstract = {Emerging evidence indicates that gene expression in higher organisms is regulated by RNA polymerase II stalling during early transcription elongation. To probe the mechanisms responsible for this regulation, we developed methods to isolate and characterize short RNAs derived from stalled RNA polymerase II in Drosophila cells. Significant levels of these short RNAs were generated from over one-third of all genes, indicating that promoter-proximal stalling is a general feature of early polymerase elongation. Nucleotide composition of the initially transcribed sequence played an important role in promoting transcriptional stalling by rendering polymerase elongation complexes highly susceptible to backtracking and arrest. These results indicate that the intrinsic efficiency of early elongation can greatly affect gene expression. }, added-at = {2009-12-13T00:28:53.000+0100}, author = {Nechaev, Sergei and Fargo, David C. and dos Santos, Gilberto and Liu, Liwen and Gao, Yuan and Adelman, Karen}, biburl = {https://www.bibsonomy.org/bibtex/273cac8ff3d60bf2ab2a3980b978ec1e8/peter.ralph}, description = {Global Analysis of Short RNAs Reveals Widespread Promoter-Proximal Stalling and Arrest of Pol II in Drosophila -- Nechaev et al., 10.1126/science.1181421 -- Science}, doi = {10.1126/science.1181421}, interhash = {2f58d678d1a66d564c9fce3fef01d95f}, intrahash = {73cac8ff3d60bf2ab2a3980b978ec1e8}, journal = {Science}, keywords = {stalled_transcription transcription}, pages = {science.1181421}, timestamp = {2009-12-13T00:28:53.000+0100}, title = {{Global Analysis of Short RNAs Reveals Widespread Promoter-Proximal Stalling and Arrest of Pol II in Drosophila}}, url = {http://www.sciencemag.org/cgi/content/abstract/science.1181421v1}, year = 2009 }