%0 Journal Article %1 zhou2021optogenetic %A Zhou, Y. %A Ding, M. %A Gao, S. %A Yu-Strzelczyk, J. %A Krischke, M. %A Duan, X. %A Leide, J. %A Riederer, M. %A Mueller, M. J. %A Hedrich, R. %A Konrad, K. R. %A Nagel, G. %D 2021 %J Nat Plants %K Optogenetics/*methods myOwn uni_network %N 2 %P 144-151 %R 10.1038/s41477-021-00853-w %T Optogenetic control of plant growth by a microbial rhodopsin %U https://www.ncbi.nlm.nih.gov/pubmed/33594268https://www.nature.com/articles/s41477-021-00853-w %V 7 %X While rhodopsin-based optogenetics has revolutionized neuroscience(1,2), poor expression of opsins and the absence of the essential cofactor all-trans-retinal has complicated the application of rhodopsins in plants. Here, we demonstrate retinal production in plants and improved rhodopsin targeting for green light manipulation of plant cells using the Guillardia theta light-gated anion channelrhodopsin GtACR1(3). Green light induces a massive increase in anion permeability and pronounced membrane potential changes when GtACR1 is expressed, enabling non-invasive manipulation of plant growth and leaf development. Using light-driven anion loss, we could mimic drought conditions and bring about leaf wilting despite sufficient water supply. Expressed in pollen tubes, global GtACR1 activation triggers membrane potential depolarizations due to large anion currents. While global illumination was associated with a reversible growth arrest, local GtACR1 activation at the flanks of the apical dome steers growth direction away from the side with increased anion conductance. These results suggest a crucial role of anion permeability for the guidance of pollen tube tip growth. This plant optogenetic approach could be expanded to create an entire pallet of rhodopsin-based tools(4), greatly facilitating dissection of plant ion-signalling pathways.

@article{zhou2021optogenetic, abstract = {While rhodopsin-based optogenetics has revolutionized neuroscience(1,2), poor expression of opsins and the absence of the essential cofactor all-trans-retinal has complicated the application of rhodopsins in plants. Here, we demonstrate retinal production in plants and improved rhodopsin targeting for green light manipulation of plant cells using the Guillardia theta light-gated anion channelrhodopsin GtACR1(3). Green light induces a massive increase in anion permeability and pronounced membrane potential changes when GtACR1 is expressed, enabling non-invasive manipulation of plant growth and leaf development. Using light-driven anion loss, we could mimic drought conditions and bring about leaf wilting despite sufficient water supply. Expressed in pollen tubes, global GtACR1 activation triggers membrane potential depolarizations due to large anion currents. While global illumination was associated with a reversible growth arrest, local GtACR1 activation at the flanks of the apical dome steers growth direction away from the side with increased anion conductance. These results suggest a crucial role of anion permeability for the guidance of pollen tube tip growth. This plant optogenetic approach could be expanded to create an entire pallet of rhodopsin-based tools(4), greatly facilitating dissection of plant ion-signalling pathways.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Zhou, Y. and Ding, M. and Gao, S. and Yu-Strzelczyk, J. and Krischke, M. and Duan, X. and Leide, J. and Riederer, M. and Mueller, M. J. and Hedrich, R. and Konrad, K. R. and Nagel, G.}, biburl = {https://www.bibsonomy.org/bibtex/24c9ded1f8d63a9c50890ba7a20cd3d73/jvsi_all}, doi = {10.1038/s41477-021-00853-w}, interhash = {2f638fdf056d28afafaf305f59c1e9d1}, intrahash = {4c9ded1f8d63a9c50890ba7a20cd3d73}, issn = {2055-0278 (Electronic) 2055-0278 (Linking)}, journal = {Nat Plants}, keywords = {Optogenetics/*methods myOwn uni_network}, note = {Zhou, Yang Ding, Meiqi Gao, Shiqiang Yu-Strzelczyk, Jing Krischke, Markus Duan, Xiaodong Leide, Jana Riederer, Markus Mueller, Martin J Hedrich, Rainer Konrad, Kai R Nagel, Georg eng England 2021/02/18 Nat Plants. 2021 Feb;7(2):144-151. doi: 10.1038/s41477-021-00853-w. Epub 2021 Feb 16.}, number = 2, pages = {144-151}, timestamp = {2024-02-15T15:11:55.000+0100}, title = {Optogenetic control of plant growth by a microbial rhodopsin}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/33594268https://www.nature.com/articles/s41477-021-00853-w}, volume = 7, year = 2021 }