Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.
Description
Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.
%0 Journal Article
%1 Schmieder:1992:Nucleic-Acids-Res:1408787
%A Schmieder, P
%A Ippel, J H
%A van den Elst, H
%A van der Marel, G A
%A van Boom, J H
%A Altona, C
%A Kessler, H
%D 1992
%J Nucleic Acids Res
%K DNA NMR
%N 18
%P 4747-4751
%T Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants
%U http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1408787
%V 20
%X Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.
@article{Schmieder:1992:Nucleic-Acids-Res:1408787,
abstract = {Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.},
added-at = {2007-12-03T10:43:17.000+0100},
author = {Schmieder, P and Ippel, J H and van den Elst, H and van der Marel, G A and van Boom, J H and Altona, C and Kessler, H},
biburl = {https://www.bibsonomy.org/bibtex/2f724cf303e532f382bacd555e5f66850/rstenutz},
description = {Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.},
interhash = {af490c25c4fe31a5ad93199e5ee24f1f},
intrahash = {f724cf303e532f382bacd555e5f66850},
journal = {Nucleic Acids Res},
keywords = {DNA NMR},
month = Sep,
number = 18,
pages = {4747-4751},
pmid = {1408787},
timestamp = {2007-12-03T10:44:28.000+0100},
title = {Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants},
url = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1408787},
volume = 20,
year = 1992
}