A new kind of chloride channels in the cardiac sarcoplasmic reticulum,
116 pS Cl$^-$ channel (500 mM Cl$^-$ in the cis and 50 mM
Cl$^-$ in the trans chamber solutions), which is activated by
protein-kinase-A-dependent phosphorylation, has been determined to
conduct adenine nucleotide as a transporter between cytosol and SR
lumen. We investigated the voltage-dependent gating of this Cl$^-$
channel by recording single-channel activities using the planar lipid
bilayer-vesicle fusion technique. The channel activities did not
change at different membrane potentials (-100 mV to +50 mV) or different
Ca$^2+$ concentrations (1 nM to 1 mM) in cis solution. In the
presence of calmodulin (CaM) (0.1 microM /microg SR vesicles), however,
Ca$^2+$ added to the cis solution at 0 mV inhibited channel openings
in a Ca$^2+$ -concentration-dependent manner. These effects were
prevented by the addition of CaM inhibitors. The blocking effects
of CaM differed depending on the membrane potentials at negative
potentials below -20 mV. With CaM and 3 microM Ca$^2+$, the values
of opening probability were 0 at -80 mV, 0.2 at -40 mV, 0.3 at -20
mV, 0.71 at 0 mV and 0.92 at +20 mV. These results may indicate the
membrane potential affects the action of Ca$^2+$ /CaM complex
%0 Journal Article
%1 Kawa_2000_189
%A Kawano, S.
%A Hiraoka, M.
%D 2000
%J Receptors Channels
%K ATPase, Acidosis, Action Adipocytes, Adolescent, Adrenergic Adult, Agents, Animals, Anti-Arrhythmia Arrhythmia, Atrial, Base Biological Biological, Block, Blockers, Body Bone Bundle-Branch COS Calcium Calcium, Calcium-Activated, Calmodulin, Cardiac, Cardiomyopathies, Cation Cell Cells, Channel Channels, Child, Chloride Comparative Concentration, Conduction Conductivity, Confocal, Cultured, Cytoplasmic DNA DNA-Binding Defects, Differentiation, Dominant, Electric Electrocardiography, Electromagnetic Electrophysiology, Endocardium, Endoplasmic Enhancement, Female, Fields, Frameshift G-Protein-Coupled, Gating, Genes, Hamsters, Heart Heart, Hematopoietic Humans, Hydrogen-Ion Image Imidazoles, Ion Isoproterenol, Kinetics, L-Type, Lidocaine, Line, Lipase, Lipoprotein Long Mapping, Markers, Marrow Membrane, Mutation, Nuclear, Nucleus, Potential Potentials, Primers, Proteins, Reticulum, Septal Sequence, Signaling, Size, Stem Study, Surface System, Transport Ventricles, and beta-Agonists, {C}a$^{2+}$-Transporting
%N 3
%P 189--197
%T Voltage-dependent effects of Ca$^2+$/calmodulin on Cl$^-$ channel
in cardiac sarcoplasmic reticulum.
%V 7
%X A new kind of chloride channels in the cardiac sarcoplasmic reticulum,
116 pS Cl$^-$ channel (500 mM Cl$^-$ in the cis and 50 mM
Cl$^-$ in the trans chamber solutions), which is activated by
protein-kinase-A-dependent phosphorylation, has been determined to
conduct adenine nucleotide as a transporter between cytosol and SR
lumen. We investigated the voltage-dependent gating of this Cl$^-$
channel by recording single-channel activities using the planar lipid
bilayer-vesicle fusion technique. The channel activities did not
change at different membrane potentials (-100 mV to +50 mV) or different
Ca$^2+$ concentrations (1 nM to 1 mM) in cis solution. In the
presence of calmodulin (CaM) (0.1 microM /microg SR vesicles), however,
Ca$^2+$ added to the cis solution at 0 mV inhibited channel openings
in a Ca$^2+$ -concentration-dependent manner. These effects were
prevented by the addition of CaM inhibitors. The blocking effects
of CaM differed depending on the membrane potentials at negative
potentials below -20 mV. With CaM and 3 microM Ca$^2+$, the values
of opening probability were 0 at -80 mV, 0.2 at -40 mV, 0.3 at -20
mV, 0.71 at 0 mV and 0.92 at +20 mV. These results may indicate the
membrane potential affects the action of Ca$^2+$ /CaM complex
@article{Kawa_2000_189,
abstract = {A new kind of chloride channels in the cardiac sarcoplasmic reticulum,
116 pS {C}l$^{-}$ channel (500 mM {C}l$^{-}$ in the cis and 50 mM
{C}l$^{-}$ in the trans chamber solutions), which is activated by
protein-kinase-A-dependent phosphorylation, has been determined to
conduct adenine nucleotide as a transporter between cytosol and SR
lumen. We investigated the voltage-dependent gating of this {C}l$^{-}$
channel by recording single-channel activities using the planar lipid
bilayer-vesicle fusion technique. The channel activities did not
change at different membrane potentials (-100 mV to +50 mV) or different
{C}a$^{2+}$ concentrations (1 nM to 1 mM) in cis solution. In the
presence of calmodulin (CaM) (0.1 microM /microg SR vesicles), however,
{C}a$^{2+}$ added to the cis solution at 0 mV inhibited channel openings
in a {C}a$^{2+}$ -concentration-dependent manner. These effects were
prevented by the addition of CaM inhibitors. The blocking effects
of CaM differed depending on the membrane potentials at negative
potentials below -20 mV. With CaM and 3 microM {C}a$^{2+}$, the values
of opening probability were 0 at -80 mV, 0.2 at -40 mV, 0.3 at -20
mV, 0.71 at 0 mV and 0.92 at +20 mV. These results may indicate the
membrane potential affects the action of {C}a$^{2+}$ /CaM complex},
added-at = {2009-06-03T11:20:58.000+0200},
author = {Kawano, S. and Hiraoka, M.},
biburl = {https://www.bibsonomy.org/bibtex/2f9211dc5fc7fcf3aaa9f85f6cbb3f670/hake},
description = {The whole bibliography file I use.},
interhash = {f24b584a6168528a686aae2c24631791},
intrahash = {f9211dc5fc7fcf3aaa9f85f6cbb3f670},
journal = {Receptors Channels},
key = 204,
keywords = {ATPase, Acidosis, Action Adipocytes, Adolescent, Adrenergic Adult, Agents, Animals, Anti-Arrhythmia Arrhythmia, Atrial, Base Biological Biological, Block, Blockers, Body Bone Bundle-Branch COS Calcium Calcium, Calcium-Activated, Calmodulin, Cardiac, Cardiomyopathies, Cation Cell Cells, Channel Channels, Child, Chloride Comparative Concentration, Conduction Conductivity, Confocal, Cultured, Cytoplasmic DNA DNA-Binding Defects, Differentiation, Dominant, Electric Electrocardiography, Electromagnetic Electrophysiology, Endocardium, Endoplasmic Enhancement, Female, Fields, Frameshift G-Protein-Coupled, Gating, Genes, Hamsters, Heart Heart, Hematopoietic Humans, Hydrogen-Ion Image Imidazoles, Ion Isoproterenol, Kinetics, L-Type, Lidocaine, Line, Lipase, Lipoprotein Long Mapping, Markers, Marrow Membrane, Mutation, Nuclear, Nucleus, Potential Potentials, Primers, Proteins, Reticulum, Septal Sequence, Signaling, Size, Stem Study, Surface System, Transport Ventricles, and beta-Agonists, {C}a$^{2+}$-Transporting},
number = 3,
pages = {189--197},
pii = {I392J001004},
pmid = {11342387},
timestamp = {2009-06-03T11:21:17.000+0200},
title = {Voltage-dependent effects of {C}a$^{2+}$/calmodulin on {C}l$^-$ channel
in cardiac sarcoplasmic reticulum.},
volume = 7,
year = 2000
}