%0 Journal Article %1 Nogales2013Toward %A Nogales, Juan %A Gudmundsson, Steinn %A Thiele, Ines %D 2013 %J Bioengineered %K cyanobacterium metabolic-engineering %N 3 %P 158--163 %R 10.4161/bioe.22792 %T Toward systems metabolic engineering in cyanobacteria: opportunities and bottlenecks. %U http://dx.doi.org/10.4161/bioe.22792 %V 4 %X We recently assessed the metabolism of Synechocystis sp PCC6803 through a constraints-based reconstruction and analysis approach and identified its main metabolic properties. These include reduced metabolic robustness, in contrast to a high photosynthetic robustness driving the optimal autotrophic metabolism. Here, we address how these metabolic features affect biotechnological capabilities of this bacterium. The search for growth-coupled overproducer strains revealed that the carbon flux re-routing, but not the electron flux, is significantly more challenging under autotrophic conditions than under mixo- or heterotrophic conditions. We also found that the blocking of the light-driven metabolism was required for carbon flux re-routing under mixotrophic conditions. Overall, our analysis, which represents the first systematic evaluation of the biotechnological capabilities of a photosynthetic organism, paradoxically suggests that the light-driven metabolism itself and its unique metabolic features are the main bottlenecks in harnessing the biotechnological potential of Synechocystis.

@article{Nogales2013Toward, abstract = {We recently assessed the metabolism of Synechocystis sp {PCC6803} through a constraints-based reconstruction and analysis approach and identified its main metabolic properties. These include reduced metabolic robustness, in contrast to a high photosynthetic robustness driving the optimal autotrophic metabolism. Here, we address how these metabolic features affect biotechnological capabilities of this bacterium. The search for growth-coupled overproducer strains revealed that the carbon flux re-routing, but not the electron flux, is significantly more challenging under autotrophic conditions than under mixo- or heterotrophic conditions. We also found that the blocking of the light-driven metabolism was required for carbon flux re-routing under mixotrophic conditions. Overall, our analysis, which represents the first systematic evaluation of the biotechnological capabilities of a photosynthetic organism, paradoxically suggests that the light-driven metabolism itself and its unique metabolic features are the main bottlenecks in harnessing the biotechnological potential of Synechocystis.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Nogales, Juan and Gudmundsson, Steinn and Thiele, Ines}, biburl = {https://www.bibsonomy.org/bibtex/2ff9059d61739964b7d2c58adf6af27a0/karthikraman}, citeulike-article-id = {12619070}, citeulike-linkout-0 = {http://dx.doi.org/10.4161/bioe.22792}, citeulike-linkout-1 = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669157/}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/23138691}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=23138691}, doi = {10.4161/bioe.22792}, interhash = {cd12078e201d70183b604cce61684136}, intrahash = {ff9059d61739964b7d2c58adf6af27a0}, issn = {2165-5987}, journal = {Bioengineered}, keywords = {cyanobacterium metabolic-engineering}, number = 3, pages = {158--163}, pmcid = {PMC3669157}, pmid = {23138691}, posted-at = {2013-09-13 07:45:34}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Toward systems metabolic engineering in cyanobacteria: opportunities and bottlenecks.}, url = {http://dx.doi.org/10.4161/bioe.22792}, volume = 4, year = 2013 }