Аннотация
We show that combined multimodal nonlinear optical (NLO) microscopies,
including two-photon excitation fluorescence, second-harmonic generation
(SHG), third harmonic generation, and fluorescence lifetime imaging
microscopy (FLIM) can be used to detect morphological and metabolic
changes associated with stroma and epithelial transformation during the
progression of cancer and osteogenesis imperfecta (01) disease. NLO
microscopes provide complementary information about tissue
microstructure, showing distinctive patterns for different types of
human breast cancer, mucinous ovarian tumors, and skin dermis of
patients with OI. Using a set of scoring methods (anisotropy,
correlation, uniformity, entropy, and lifetime components), we found
significant differences in the content, distribution and organization of
collagen fibrils in the stroma of breast and ovary as well as in the
dermis of skin. We suggest that our results provide a framework for
using NLO techniques as a clinical diagnostic tool for human cancer and
OI. We further suggest that the SHG and FLIM metrics described could be
applied to other connective or epithelial tissue disorders that are
characterized by abnormal cells proliferation and collagen assembly. (C)
2012 Society of Photo-Optical Instrumentation Engineers (SPIE). DOI:
10.1117/1.JBO.17.8.081407
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