%0 Journal Article %1 citeulike:708240 %A Kuhlman, B. %A Dantas, G. %A Ireton, G. C. %A Varani, G. %A Stoddard, B. L. %A Baker, D. %C Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. %D 2003 %J Science %K design, post-defense, predictions, rosetta, structure %N 5649 %P 1364--1368 %R 10.1126/science.1089427 %T Design of a novel globular protein fold with atomic-level accuracy. %U http://dx.doi.org/10.1126/science.1089427 %V 302 %X A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue alpha/beta protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature.

@article{citeulike:708240, abstract = {A major challenge of computational protein design is the creation of novel proteins with arbitrarily chosen three-dimensional structures. Here, we used a general computational strategy that iterates between sequence design and structure prediction to design a 93-residue alpha/beta protein called Top7 with a novel sequence and topology. Top7 was found experimentally to be folded and extremely stable, and the x-ray crystal structure of Top7 is similar (root mean square deviation equals 1.2 angstroms) to the design model. The ability to design a new protein fold makes possible the exploration of the large regions of the protein universe not yet observed in nature.}, added-at = {2009-05-19T18:00:18.000+0200}, address = {Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.}, author = {Kuhlman, B. and Dantas, G. and Ireton, G. C. and Varani, G. and Stoddard, B. L. and Baker, D.}, biburl = {https://www.bibsonomy.org/bibtex/226f9b2ba6f2861a26b2ea734302bb2a6/earthfare}, citeulike-article-id = {708240}, description = {CiteULike: Everyone's library}, doi = {10.1126/science.1089427}, interhash = {34af90061fd6e912b464fddd5e64e7a8}, intrahash = {26f9b2ba6f2861a26b2ea734302bb2a6}, issn = {1095-9203}, journal = {Science}, keywords = {design, post-defense, predictions, rosetta, structure}, month = {November}, number = 5649, pages = {1364--1368}, posted-at = {2009-05-19 04:23:35}, priority = {2}, timestamp = {2009-05-19T18:03:27.000+0200}, title = {Design of a novel globular protein fold with atomic-level accuracy.}, url = {http://dx.doi.org/10.1126/science.1089427}, volume = 302, year = 2003 }