%0 Journal Article %1 Mirsaleh-Kohan2010 %A Mirsaleh-Kohan, Nasrin %A Bass, Andrew D %A Sanche, Léon %D 2010 %J Langmuir %K chemistry/isolation/&/purification;SurfaceProperties;UltravioletRays chemistry;Oligodeoxyribonucleotides Adsorption;BaseSequence;DNA chemistry/genetics;Ozone Single-Stranded chemistry;Sulfur chemistry;Hydroxides chemistry;Phosphates chemistry;PhotoelectronSpectroscopy;SulfhydrylCompounds chemistry/genetics;Gold %N 9 %P 6508--6514 %R 10.1021/la9039804 %T X-ray photoelectron spectroscopy analysis of gold surfaces after removal of thiolated DNA oligomers by ultraviolet/ozone treatment. %U http://dx.doi.org/10.1021/la9039804 %V 26 %X Well-ordered films of molecular DNA can be formed by the attachment of thiolated DNA oligonucleotides to a supporting gold substrate. The gold substrate represents a significant fraction of the total cost of preparing such films, and it is thus important to determine whether such substrates can be reused. Here, we investigate with X-ray photoelectron spectroscopy the suitability of UV/ozonolysis previously employed to remove alkanethiols from gold, for removing 40-mer, single- and double-stranded synthetic DNA. We find that while UV/O(3) can indeed remove thiolated DNA from gold slides, the treatment times required permit the implantation of additional organic contaminants.

@article{Mirsaleh-Kohan2010, abstract = {Well-ordered films of molecular DNA can be formed by the attachment of thiolated DNA oligonucleotides to a supporting gold substrate. The gold substrate represents a significant fraction of the total cost of preparing such films, and it is thus important to determine whether such substrates can be reused. Here, we investigate with X-ray photoelectron spectroscopy the suitability of UV/ozonolysis previously employed to remove alkanethiols from gold, for removing 40-mer, single- and double-stranded synthetic DNA. We find that while UV/O(3) can indeed remove thiolated DNA from gold slides, the treatment times required permit the implantation of additional organic contaminants.}, added-at = {2011-07-21T21:36:16.000+0200}, author = {Mirsaleh-Kohan, Nasrin and Bass, Andrew D and Sanche, Léon}, biburl = {https://www.bibsonomy.org/bibtex/27762918fda2f07c5b94774ea1c6f57c8/crc_chus}, doi = {10.1021/la9039804}, institution = {Département de médecine nucléaire et radiobiologie, Faculté de médecine et les sciences de santé, Université de Sherbrooke, Sherbrooke, QC Canada J1H 5N4. nasrin.mirsaleh@usherbrooke.ca}, interhash = {350f88d42f9b6cad8dd32191e1a124ad}, intrahash = {7762918fda2f07c5b94774ea1c6f57c8}, journal = {Langmuir}, keywords = {chemistry/isolation/&/purification;SurfaceProperties;UltravioletRays chemistry;Oligodeoxyribonucleotides Adsorption;BaseSequence;DNA chemistry/genetics;Ozone Single-Stranded chemistry;Sulfur chemistry;Hydroxides chemistry;Phosphates chemistry;PhotoelectronSpectroscopy;SulfhydrylCompounds chemistry/genetics;Gold}, language = {eng}, medline-pst = {ppublish}, month = May, number = 9, pages = {6508--6514}, pmid = {20000594}, timestamp = {2011-07-21T21:36:16.000+0200}, title = {X-ray photoelectron spectroscopy analysis of gold surfaces after removal of thiolated DNA oligomers by ultraviolet/ozone treatment.}, url = {http://dx.doi.org/10.1021/la9039804}, volume = 26, year = 2010 }