Аннотация
Disclosed are methods that permit the determination of both lipid moiety (LM) and apolipoprotein expressed epitope immunoreactivity (EEI) of intact isolated lipoprotein species. One format of the method isolates a lipoprotein species by means of a ligand binding to a solid phase, and quantitates thereafter the LM and/or the EEI. A complementary variant format provides for nonquantitative study of a constant number of lipoprotein particles exploring the sizes of the particles (LM) and scanning the apolipoprotein epitopes (EEI). Disclosed also is a method using lipid moiety dynamics (LMD) and expressed epitopes immunoreactivity dynamics (EEID) to explore the lipid loading and unloading of the lipoprotein species during a fast-feeding cycle. Both the LMD and EEID of lipoprotein particles may be performed as a panel, individual or in any combination. This can be used to detect abnormalities in lipid metabolism that precede the hyperlipidemias associated with cardiovascular disease.
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