%0 Journal Article %1 Higgins.1994 %A Higgins, M. J. %A Smilinich, N. J. %A Sait, S. %A Koenig, A. %A Pongratz, J. %A Gessler, M. %A Richard, C. W. 3rd %A James, M. R. %A Sanford, J. P. %A Kim, B. W. %A Cattelane, J. %A Nowak, N. J. %A Winterpacht, A. %A Zabel, B. U. %A Munroe, D. J. %A Bric, E. %A Housman, D. E. %A Jones, C. %A Nakamura, Y. %A S., Gerhard D. %A Shows, T. B. %D 1994 %J Genomics %K *Chromosomes;Human;Pair *Deoxyribonucleases;Type *Restriction 11 Cells Cells;Cultured Chromosome Cosmids DNA Deletion Electrophoresis;Gel;Pulsed-Field Humans Hybrid II Mapping Probes Sequence Site-Specific %N 1 %P 211--222 %T An ordered NotI fragment map of human chromosome band 11p15 %V 23 %X An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85\% of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH-CALCA-ST5-RBTN1-HPX-HBB-RRM1 -TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS - RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.

@article{Higgins.1994, abstract = {An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85{\%} of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH-CALCA-ST5-RBTN1-HPX-HBB-RRM1 -TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS - RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.}, added-at = {2013-01-29T13:47:26.000+0100}, author = {Higgins, M. J. and Smilinich, N. J. and Sait, S. and Koenig, A. and Pongratz, J. and Gessler, M. and Richard, C. W. 3rd and James, M. R. and Sanford, J. P. and Kim, B. W. and Cattelane, J. and Nowak, N. J. and Winterpacht, A. and Zabel, B. U. and Munroe, D. J. and Bric, E. and Housman, D. E. and Jones, C. and Nakamura, Y. and S., Gerhard D. and Shows, T. B.}, biburl = {https://www.bibsonomy.org/bibtex/295e51c2be8bcf4cd13523b0b4bb7aa06/ebch}, interhash = {3f8dc81f12b2a4278983c41d458d2262}, intrahash = {95e51c2be8bcf4cd13523b0b4bb7aa06}, journal = {Genomics}, keywords = {*Chromosomes;Human;Pair *Deoxyribonucleases;Type *Restriction 11 Cells Cells;Cultured Chromosome Cosmids DNA Deletion Electrophoresis;Gel;Pulsed-Field Humans Hybrid II Mapping Probes Sequence Site-Specific}, number = 1, pages = {211--222}, timestamp = {2013-01-29T13:47:41.000+0100}, title = {An ordered NotI fragment map of human chromosome band 11p15}, volume = 23, year = 1994 }