%0 Journal Article %1 ekins1989development %A Ekins, Roger %A Chu, Frederick %A Biggart, Elizabeth %D 1989 %J Analytica Chimica Acta %K immunoassay %N 0 %P 73 - 96 %R 10.1016/S0003-2670(00)82647-1 %T Development of microspot multi-analyte ratiometric immunoassay using dual fluorescent-labelled antibodies %U http://www.sciencedirect.com/science/article/pii/S0003267000826471 %V 227 %X Radioisotopes are progressively being replaced by non-isotopic labels as reagent markers for immunoassay purposes. The reasons for this development include an avoidance of the practical and environmental disadvantages associated with the use of radioisotopes and the need for higher assay sensitivity. A further stimulus is the increasing requirement to measure multiple analytes simultaneously in the same sample. This paper outlines the general principles underlying multi-analyte “microspot” immunoassay methodology. It relies on the measurement of the ratio of fluorescent signals from individual antibody “microspots” forming a microspot array on a suitable plastic surface. In principle, the methodology is capable of measuring 106 different substances in a sample volume of 100 μl.

@article{ekins1989development, abstract = {Radioisotopes are progressively being replaced by non-isotopic labels as reagent markers for immunoassay purposes. The reasons for this development include an avoidance of the practical and environmental disadvantages associated with the use of radioisotopes and the need for higher assay sensitivity. A further stimulus is the increasing requirement to measure multiple analytes simultaneously in the same sample. This paper outlines the general principles underlying multi-analyte “microspot” immunoassay methodology. It relies on the measurement of the ratio of fluorescent signals from individual antibody “microspots” forming a microspot array on a suitable plastic surface. In principle, the methodology is capable of measuring 106 different substances in a sample volume of 100 μl.}, added-at = {2013-02-04T16:16:25.000+0100}, author = {Ekins, Roger and Chu, Frederick and Biggart, Elizabeth}, biburl = {https://www.bibsonomy.org/bibtex/20031bc94707b8a4d0598c80c22fa7969/vch}, description = {ScienceDirect.com - Analytica Chimica Acta - Development of microspot multi-analyte ratiometric immunoassay using dual fluorescent-labelled antibodies}, doi = {10.1016/S0003-2670(00)82647-1}, interhash = {4022780af2c6eaa4cd29d02f8ffce50f}, intrahash = {0031bc94707b8a4d0598c80c22fa7969}, issn = {0003-2670}, journal = {Analytica Chimica Acta}, keywords = {immunoassay}, number = 0, pages = {73 - 96}, timestamp = {2013-02-04T16:16:25.000+0100}, title = {Development of microspot multi-analyte ratiometric immunoassay using dual fluorescent-labelled antibodies}, url = {http://www.sciencedirect.com/science/article/pii/S0003267000826471}, volume = 227, year = 1989 }