%0 Journal Article %1 citeulike:470763 %A Kaptein, Wilhelmina A. %A Kor, %A Venema, %A Ruiters, Marcel H. %A Korf, Jakob %D 1997 %J Sensors and Actuators B: Chemical %K displacement immunoassay ag_detection electrochemistry %N 1 %P 63--69 %R 10.1016/S0925-4005(97)00271-2 %T Analysis of cortisol with a flow displacement immunoassay %U http://www.sciencedirect.com/science/article/B6THH-3S72MPH-9/2/6c0f90083b1bfc98a6345e7129480900 %V 45 %X This study investigates a flow immunoassay for cortisol based on displacement of labelled antigen analogues from immobilised antibodies. On a displacement column, two antibodies with different affinities were immobilised on protein-A Sepharose, and a construct of cortisol and horseradish peroxidase (cort-HRP) was associated to the antibodies. Perfusion of the column with cortisol displaced a proportional amount of cort-HRP. On-line detection was performed with an electrochemical detector measuring cort-HRP directly in a flow injection system. For investigating some characteristics of the displacement system, cort-HRP was also detected off-line, with a substrate for HRP, which changes OD492. We tested our system in buffer as well in serum for 2 days at a flow of 4-6 mul min-1. Analysis of the data show a detectable displacement signal at physiological cortisol concentrations. The specificity, stability of the columns and the influence of the antibody affinity were studied.

@article{citeulike:470763, abstract = {This study investigates a flow immunoassay for cortisol based on displacement of labelled antigen analogues from immobilised antibodies. On a displacement column, two antibodies with different affinities were immobilised on protein-A Sepharose, and a construct of cortisol and horseradish peroxidase (cort-HRP) was associated to the antibodies. Perfusion of the column with cortisol displaced a proportional amount of cort-HRP. On-line detection was performed with an electrochemical detector measuring cort-HRP directly in a flow injection system. For investigating some characteristics of the displacement system, cort-HRP was also detected off-line, with a substrate for HRP, which changes OD492. We tested our system in buffer as well in serum for 2 days at a flow of 4-6 [mu]l min-1. Analysis of the data show a detectable displacement signal at physiological cortisol concentrations. The specificity, stability of the columns and the influence of the antibody affinity were studied.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Kaptein, Wilhelmina A. and Kor and Venema and Ruiters, Marcel H. and Korf, Jakob}, biburl = {https://www.bibsonomy.org/bibtex/2061f7c9a6bd2232d53baa87ea679c8cd/biblio24}, citeulike-article-id = {470763}, comment = {W.A.Kaptein@MED.RUG.NL --- PDF}, doi = {10.1016/S0925-4005(97)00271-2}, interhash = {4437093c0a27d3b379bfa4dfbd1adaf1}, intrahash = {061f7c9a6bd2232d53baa87ea679c8cd}, journal = {Sensors and Actuators B: Chemical}, keywords = {displacement immunoassay ag_detection electrochemistry}, month = {November}, number = 1, pages = {63--69}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Analysis of cortisol with a flow displacement immunoassay}, url = {http://www.sciencedirect.com/science/article/B6THH-3S72MPH-9/2/6c0f90083b1bfc98a6345e7129480900}, volume = 45, year = 1997 }