%0 Journal Article %1 doi:10.1021/ja049351u %A Heilemann, Mike %A Tinnefeld, Philip %A Mosteiro, Gabriel Sanchez %A Parajo, Maria Garcia %A Hulst, Niek F. Van %A Sauer, Markus %D 2004 %J Journal of the American Chemical Society %K mike sauer %N 21 %P 6514-6515 %R 10.1021/ja049351u %T Multistep Energy Transfer in Single Molecular Photonic Wires %U http://pubs.acs.org/doi/abs/10.1021/ja049351u %V 126 %X We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various chromophores at well-defined positions and arbitrary interchromophore distances. While ensemble fluorescence measurements show overall FRET efficiencies between 15 and 30%, single-molecule spectroscopy performed on four spectrally separated detectors easily uncovers subpopulations that exhibit overall FRET efficiencies of up to 90% across a distance of 13.6 nm and a spectral range of 200 nm. Fluorescence trajectories of individual photonic wires show five different fluorescence intensity patterns which can be ascribed to successive photobleaching events

@article{doi:10.1021/ja049351u, abstract = {We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various chromophores at well-defined positions and arbitrary interchromophore distances. While ensemble fluorescence measurements show overall FRET efficiencies between 15 and 30%, single-molecule spectroscopy performed on four spectrally separated detectors easily uncovers subpopulations that exhibit overall FRET efficiencies of up to 90% across a distance of 13.6 nm and a spectral range of 200 nm. Fluorescence trajectories of individual photonic wires show five different fluorescence intensity patterns which can be ascribed to successive photobleaching events}, added-at = {2011-03-15T10:21:16.000+0100}, author = {Heilemann, Mike and Tinnefeld, Philip and Mosteiro, Gabriel Sanchez and Parajo, Maria Garcia and Hulst, Niek F. Van and Sauer, Markus}, biburl = {https://www.bibsonomy.org/bibtex/2ca5eb9809767b3b73d9bc56b545d045a/reichert}, doi = {10.1021/ja049351u}, eprint = {http://pubs.acs.org/doi/pdf/10.1021/ja049351u}, interhash = {4517ded25492dfe16e058aa6b3cc479b}, intrahash = {ca5eb9809767b3b73d9bc56b545d045a}, journal = {Journal of the American Chemical Society}, keywords = {mike sauer}, number = 21, pages = {6514-6515}, timestamp = {2011-03-15T10:21:16.000+0100}, title = {Multistep Energy Transfer in Single Molecular Photonic Wires}, url = {http://pubs.acs.org/doi/abs/10.1021/ja049351u}, volume = 126, year = 2004 }