%0 Journal Article %1 Yus2009Impact %A Yus, Eva %A Maier, Tobias %A Michalodimitrakis, Konstantinos %A van Noort, Vera %A Yamada, Takuji %A Chen, Wei-Hua H. %A Wodke, Judith A. %A Güell, Marc %A Mart\'ınez, Sira %A Bourgeois, Ronan %A Kühner, Sebastian %A Raineri, Emanuele %A Letunic, Ivica %A Kalinina, Olga V. %A Rode, Michaela %A Herrmann, Richard %A Gutiérrez-Gallego, Ricardo %A Russell, Robert B. %A Gavin, Anne-Claude C. %A Bork, Peer %A Serrano, Luis %B Science %D 2009 %J Science (New York, N.Y.) %K inbi metabolism minimal-genome regulation %N 5957 %P 1263--1268 %R 10.1126/science.1177263 %T Impact of genome reduction on bacterial metabolism and its regulation. %U http://dx.doi.org/10.1126/science.1177263 %V 326 %X To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplasma pneumoniae. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and 13C-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however.

@article{Yus2009Impact, abstract = { To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplasma pneumoniae. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and {13C}-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however. }, added-at = {2018-12-02T16:09:07.000+0100}, author = {Yus, Eva and Maier, Tobias and Michalodimitrakis, Konstantinos and van Noort, Vera and Yamada, Takuji and Chen, Wei-Hua H. and Wodke, Judith A. and G\"{u}ell, Marc and Mart\'{\i}nez, Sira and Bourgeois, Ronan and K\"{u}hner, Sebastian and Raineri, Emanuele and Letunic, Ivica and Kalinina, Olga V. and Rode, Michaela and Herrmann, Richard and Guti\'{e}rrez-Gallego, Ricardo and Russell, Robert B. and Gavin, Anne-Claude C. and Bork, Peer and Serrano, Luis}, biburl = {https://www.bibsonomy.org/bibtex/269bbbdb6b2628e3b36a252ab2349133a/karthikraman}, booktitle = {Science}, citeulike-article-id = {6227603}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1177263}, citeulike-linkout-1 = {http://www.sciencemag.org/content/326/5957/1263.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/326/5957/1263.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/326/5957/1263}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/19965476}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=19965476}, day = 27, doi = {10.1126/science.1177263}, interhash = {45e67412f34fe5800fa262a6f1436e40}, intrahash = {69bbbdb6b2628e3b36a252ab2349133a}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {inbi metabolism minimal-genome regulation}, month = nov, number = 5957, pages = {1263--1268}, pmid = {19965476}, posted-at = {2010-05-21 15:23:29}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Impact of genome reduction on bacterial metabolism and its regulation.}, url = {http://dx.doi.org/10.1126/science.1177263}, volume = 326, year = 2009 }