%0 Journal Article %1 meng2015solubilized %A Meng, Fan Wei %A Slivka, Peter F. %A Dearth, Christopher L. %A Badylak, Stephen F. %D 2015 %J Biomaterials %K ecm phd %N 0 %P 131--140 %R http://dx.doi.org/10.1016/j.biomaterials.2014.12.044 %T Solubilized extracellular matrix from brain and urinary bladder elicits distinct functional and phenotypic responses in macrophages %U http://www.sciencedirect.com/science/article/pii/S0142961214012940 %V 46 %X Abstract Extracellular matrix (ECM) derived from a variety of source tissues has been successfully used to facilitate tissue reconstruction. The recent development of solubilized forms of \ECM\ advances the therapeutic potential of these biomaterials. Isolated, soluble components of \ECM\ and matricryptic peptides have been shown to bias macrophages toward a regulatory and constructive (M2-like) phenotype. However, the majority of studies described thus far have utilized anatomically and morphologically similar gastrointestinal derived \ECMs\ (small intestine, esophagus, urinary bladder, etc.) and a small subset of macrophage markers (CD206, CD86, CCR7) to describe them. The present study evaluated the effect of solubilized \ECM\ derived from molecularly diverse source tissues (brain and urinary bladder) upon primary macrophage phenotype and function. Results showed that solubilized urinary bladder \ECM\ (U-ECM) up-regulated macrophage \PGE2\ secretion and suppressed traditional pro-inflammatory factor secretion, consistent with an M2-like phenotype. The hyaluronic acid (HA) component in solubilized U-ECM played an important role in mediating this response. Brain \ECM\ (B-ECM) elicited a pro-inflammatory (M1-like) macrophage response and contained almost no HA. These findings suggest that the molecular composition of the source tissue \ECM\ plays an important role in influencing macrophage function and phenotype.

@article{meng2015solubilized, abstract = {Abstract Extracellular matrix (ECM) derived from a variety of source tissues has been successfully used to facilitate tissue reconstruction. The recent development of solubilized forms of \{ECM\} advances the therapeutic potential of these biomaterials. Isolated, soluble components of \{ECM\} and matricryptic peptides have been shown to bias macrophages toward a regulatory and constructive (M2-like) phenotype. However, the majority of studies described thus far have utilized anatomically and morphologically similar gastrointestinal derived \{ECMs\} (small intestine, esophagus, urinary bladder, etc.) and a small subset of macrophage markers (CD206, CD86, CCR7) to describe them. The present study evaluated the effect of solubilized \{ECM\} derived from molecularly diverse source tissues (brain and urinary bladder) upon primary macrophage phenotype and function. Results showed that solubilized urinary bladder \{ECM\} (U-ECM) up-regulated macrophage \{PGE2\} secretion and suppressed traditional pro-inflammatory factor secretion, consistent with an M2-like phenotype. The hyaluronic acid (HA) component in solubilized U-ECM played an important role in mediating this response. Brain \{ECM\} (B-ECM) elicited a pro-inflammatory (M1-like) macrophage response and contained almost no HA. These findings suggest that the molecular composition of the source tissue \{ECM\} plays an important role in influencing macrophage function and phenotype. }, added-at = {2015-02-02T15:48:53.000+0100}, author = {Meng, Fan Wei and Slivka, Peter F. and Dearth, Christopher L. and Badylak, Stephen F.}, biburl = {https://www.bibsonomy.org/bibtex/2d25e681a6075bce3b350ec8a25e44fbd/bkoch}, description = {Solubilized extracellular matrix from brain and urinary bladder elicits distinct functional and phenotypic responses in macrophages}, doi = {http://dx.doi.org/10.1016/j.biomaterials.2014.12.044}, interhash = {47bcb37e0999cc319c4b828418f2513d}, intrahash = {d25e681a6075bce3b350ec8a25e44fbd}, issn = {0142-9612}, journal = {Biomaterials }, keywords = {ecm phd}, number = 0, pages = {131--140}, timestamp = {2015-02-02T15:48:53.000+0100}, title = {Solubilized extracellular matrix from brain and urinary bladder elicits distinct functional and phenotypic responses in macrophages }, url = {http://www.sciencedirect.com/science/article/pii/S0142961214012940}, volume = 46, year = 2015 }