%0 Journal Article %1 citeulike:480433 %A Robinson, G. A. %A Martinazzo, G. %A Forrest, G. C. %D 1986 %J J Immunoassay %K displacement competition immunoassay homogeneous immunosensor ag_detection electrochemistry %N 1-2 %P 1--15 %T A homogeneous bioelectrochemical immunoassay for thyroxine. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3734106 %V 7 %X Thyroxine has been modified with a ferrocene derivative to produce an immunologically reactive conjugate which remains capable of functioning as an electron transfer mediator, shuttling electrons between an oxido-reductase enzyme and an electrode. On binding with an anti-thyroxine antibody the conjugate's ability to function as a mediator is impaired. The conjugate has been used as a tracer in an homogeneous competitive assay to quantify the total thyroxine concentration in serum, the assay being measured electrochemically after the addition of a suitable oxidoreductase enzyme (glucose oxidase) and its substrate (glucose) on completion of antibody/antigen binding. This results in a simple and sensitive assay for thyroxine.

@article{citeulike:480433, abstract = {Thyroxine has been modified with a ferrocene derivative to produce an immunologically reactive conjugate which remains capable of functioning as an electron transfer mediator, shuttling electrons between an oxido-reductase enzyme and an electrode. On binding with an anti-thyroxine antibody the conjugate's ability to function as a mediator is impaired. The conjugate has been used as a tracer in an homogeneous competitive assay to quantify the total thyroxine concentration in serum, the assay being measured electrochemically after the addition of a suitable oxidoreductase enzyme (glucose oxidase) and its substrate (glucose) on completion of antibody/antigen binding. This results in a simple and sensitive assay for thyroxine.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Robinson, G. A. and Martinazzo, G. and Forrest, G. C.}, biburl = {https://www.bibsonomy.org/bibtex/2d26a49d5ef7faf434801ebc2a2913a60/biblio24}, citeulike-article-id = {480433}, interhash = {4e64df7f65cad6cf3e32ce8e343ea6c3}, intrahash = {d26a49d5ef7faf434801ebc2a2913a60}, issn = {0197-1522}, journal = {J Immunoassay}, keywords = {displacement competition immunoassay homogeneous immunosensor ag_detection electrochemistry}, number = {1-2}, pages = {1--15}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {A homogeneous bioelectrochemical immunoassay for thyroxine.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=3734106}, volume = 7, year = 1986 }