%0 Journal Article %1 Lohse2007a %A Lohse, M. J. %A Bunemann, M. %A Hoffmann, C. %A Vilardaga, J. P. %A Nikolaev, V. O. %D 2007 %J Curr Opin Pharmacol %K *Fluorescence *Signal AMP/metabolism Animals Arrestins/metabolism Cyclic Energy G-Protein-Coupled/metabolism Humans Resonance Transduction Transfer Receptor %N 5 %P 547-53 %T Monitoring receptor signaling by intramolecular FRET %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17919975 %V 7 %X A large variety of techniques has been used to monitor activation of G protein-coupled receptors (GPCRs) both in isolated membranes and in intact cells. However, most of these techniques cannot resolve receptor activation and signaling in space and in time. Here, we describe techniques that allow the temporally and spatially resolved monitoring of these processes by optical recording with energy transfer techniques. Fluorescence and bioluminescence resonance energy transfer, FRET and BRET, are based on energy transfer between two closely spaced probes. The exquisite sensitivity of FRET and BRET to the distance of the two probes makes these techniques ideal tools to study either protein-protein interactions (when the two probes are localized on two different proteins) or conformational changes within a given protein (when the two probes are localized on a single protein). Here, we review the latter approach as a tool to study receptor activation and the levels of the second messengers cAMP and cGMP in intact cells.

@article{Lohse2007a, abstract = {A large variety of techniques has been used to monitor activation of G protein-coupled receptors (GPCRs) both in isolated membranes and in intact cells. However, most of these techniques cannot resolve receptor activation and signaling in space and in time. Here, we describe techniques that allow the temporally and spatially resolved monitoring of these processes by optical recording with energy transfer techniques. Fluorescence and bioluminescence resonance energy transfer, FRET and BRET, are based on energy transfer between two closely spaced probes. The exquisite sensitivity of FRET and BRET to the distance of the two probes makes these techniques ideal tools to study either protein-protein interactions (when the two probes are localized on two different proteins) or conformational changes within a given protein (when the two probes are localized on a single protein). Here, we review the latter approach as a tool to study receptor activation and the levels of the second messengers cAMP and cGMP in intact cells.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Lohse, M. J. and Bunemann, M. and Hoffmann, C. and Vilardaga, J. P. and Nikolaev, V. O.}, biburl = {https://www.bibsonomy.org/bibtex/27321751d174b5cc56d5facf5ef968b1d/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {4f4306268dc7f96777509f50a8998bf3}, intrahash = {7321751d174b5cc56d5facf5ef968b1d}, issn = {1471-4892 (Print) 1471-4892 (Linking)}, journal = {Curr Opin Pharmacol}, keywords = {*Fluorescence *Signal AMP/metabolism Animals Arrestins/metabolism Cyclic Energy G-Protein-Coupled/metabolism Humans Resonance Transduction Transfer Receptor}, month = Oct, note = {Lohse, Martin J Bunemann, Moritz Hoffmann, Carsten Vilardaga, Jean-Pierre Nikolaev, Viacheslav O Review England Current opinion in pharmacology Curr Opin Pharmacol. 2007 Oct;7(5):547-53. Epub 2007 Oct 4.}, number = 5, pages = {547-53}, shorttitle = {Monitoring receptor signaling by intramolecular FRET}, timestamp = {2010-12-14T18:20:06.000+0100}, title = {Monitoring receptor signaling by intramolecular FRET}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17919975}, volume = 7, year = 2007 }