%0 Journal Article %1 forsyth_determination_1994 %A Forsyth, R. J. %A Ip, D. P. %D 1994 %J Journal of Pharmaceutical and Biomedical Analysis %K Imipenem, Primaxin® cilastatin derivative sodium, spectrophotometry., {UV} %N 10 %P 1243--1248 %R 10.1016/0731-7085(94)00067-0 %T Determination of imipenem and cilastatin sodium in Primaxin® by first order derivative ultraviolet spectrophotometry %U http://www.sciencedirect.com/science/article/B6TGX-44CYSH2-3J/2/6990b98add2e23779a748db38ee3292f %V 12 %X A UV-spectrophotometric assay to measure the concentrations of the active drug components (imipenem and cilastatin) or Primaxin® for routine release testing is described. The assay is based on the use of first order derivative spectrophotometry. The trough amplitudes in the first derivative spectrophotometric spectra at 243 and 318 nm were selected to determine cilastatin and imipenem, respectively. A linear relationship (R \textgreater 0.99) between the trough amplitudes and concentrations was demonstrated over the range 14-42 mug ml-1 for both drug components. Commercial IV formulations and laboratory prepared mixtures containing both drugs in different proportions were assayed using the developed method with good recoveries (ave. 100.6\%). The method is rapid, precise, accurate and was shown to be equivalent to the more time consuming LC method; which is currently used for routine release testing. The specificity and stability indicating properties of the method will also be addressed.

@article{forsyth_determination_1994, abstract = {A {UV-spectrophotometric} assay to measure the concentrations of the active drug components (imipenem and cilastatin) or Primaxin® for routine release testing is described. The assay is based on the use of first order derivative spectrophotometry. The trough amplitudes in the first derivative spectrophotometric spectra at 243 and 318 nm were selected to determine cilastatin and imipenem, respectively. A linear relationship {(R} {\textgreater} 0.99) between the trough amplitudes and concentrations was demonstrated over the range 14-42 [mu]g ml-1 for both drug components. Commercial {IV} formulations and laboratory prepared mixtures containing both drugs in different proportions were assayed using the developed method with good recoveries (ave. 100.6\%). The method is rapid, precise, accurate and was shown to be equivalent to the more time consuming {LC} method; which is currently used for routine release testing. The specificity and stability indicating properties of the method will also be addressed.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Forsyth, R. J. and Ip, D. P.}, biburl = {https://www.bibsonomy.org/bibtex/20345d070fe99b5da1adb16414282f624/jelias}, doi = {10.1016/0731-7085(94)00067-0}, interhash = {5134d95744bd25903e437e4b2a037af9}, intrahash = {0345d070fe99b5da1adb16414282f624}, issn = {0731-7085}, journal = {Journal of Pharmaceutical and Biomedical Analysis}, keywords = {Imipenem, Primaxin® cilastatin derivative sodium, spectrophotometry., {UV}}, month = oct, number = 10, pages = {1243--1248}, timestamp = {2011-03-11T10:06:38.000+0100}, title = {Determination of imipenem and cilastatin sodium in Primaxin® by first order derivative ultraviolet spectrophotometry}, url = {http://www.sciencedirect.com/science/article/B6TGX-44CYSH2-3J/2/6990b98add2e23779a748db38ee3292f}, volume = 12, year = 1994 }