Abstract
The evaluation of the nanoparticles interactions with blood plasma
enables a first and important insight on the organization of the
adsorbed protein layer. In this context, we studied the formation of protein corona on magneto-luminescent Fe3O4@calix-Eu(TTA) nanoparticles
in human plasma. The difference in the surface chemistry of F3O4
functionalized with calixarene (+30 mV zeta-potential) and europium
(III) thenoyltrifluoroacetonate (Eu3+-TTA) complex (+7.4 mV
zeta-potential) affected the colloidal stability and hard corona
composition of the nanoparticles, which were monitored by SDS-PAGE gel
electrophoresis, differential centrifugal sedimentation analyses and
luminescence spectroscopy. Strikingly, after conjugation with Eu3+ TTA
complex, the magnetic nanoparticles show lower adsorption affinity to
proteins at higher blood plasma concentration. Moreover, the Eu3+
compound is featured with the narrow emission lines of D-5(0)-> F-7(J) (J=0-4) transitions, imparting additional characteristics to
bifunctional nanoparticles that were used as probes to evaluate the
protein corona formation.
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