%0 Journal Article %1 huang2019calcium %A Huang, S. %A Waadt, R. %A Nuhkat, M. %A Kollist, H. %A Hedrich, R. %A Roelfsema, M. R. G. %D 2019 %J New Phytol %K Abscisic Acid/*pharmacology myOwn %N 1 %P 177-187 %R 10.1111/nph.15985 %T Calcium signals in guard cells enhance the efficiency by which abscisic acid triggers stomatal closure %U https://www.ncbi.nlm.nih.gov/pubmed/31179540 %V 224 %X During drought, abscisic acid (ABA) induces closure of stomata via a signaling pathway that involves the calcium (Ca(2+) )-independent protein kinase OST1, as well as Ca(2+) -dependent protein kinases. However, the interconnection between OST1 and Ca(2+) signaling in ABA-induced stomatal closure has not been fully resolved. ABA-induced Ca(2+) signals were monitored in intact Arabidopsis leaves, which express the ratiometric Ca(2+) reporter R-GECO1-mTurquoise and the Ca(2+) -dependent activation of S-type anion channels was recorded with intracellular double-barreled microelectrodes. ABA triggered Ca(2+) signals that occurred during the initiation period, as well as in the acceleration phase of stomatal closure. However, a subset of stomata closed in the absence of Ca(2+) signals. On average, stomata closed faster if Ca(2+) signals were elicited during the ABA response. Loss of OST1 prevented ABA-induced stomatal closure and repressed Ca(2+) signals, whereas elevation of the cytosolic Ca(2+) concentration caused a rapid activation of SLAC1 and SLAH3 anion channels. Our data show that the majority of Ca(2+) signals are evoked during the acceleration phase of stomatal closure, which is initiated by OST1. These Ca(2+) signals are likely to activate Ca(2+) -dependent protein kinases, which enhance the activity of S-type anion channels and boost stomatal closure.

@article{huang2019calcium, abstract = {During drought, abscisic acid (ABA) induces closure of stomata via a signaling pathway that involves the calcium (Ca(2+) )-independent protein kinase OST1, as well as Ca(2+) -dependent protein kinases. However, the interconnection between OST1 and Ca(2+) signaling in ABA-induced stomatal closure has not been fully resolved. ABA-induced Ca(2+) signals were monitored in intact Arabidopsis leaves, which express the ratiometric Ca(2+) reporter R-GECO1-mTurquoise and the Ca(2+) -dependent activation of S-type anion channels was recorded with intracellular double-barreled microelectrodes. ABA triggered Ca(2+) signals that occurred during the initiation period, as well as in the acceleration phase of stomatal closure. However, a subset of stomata closed in the absence of Ca(2+) signals. On average, stomata closed faster if Ca(2+) signals were elicited during the ABA response. Loss of OST1 prevented ABA-induced stomatal closure and repressed Ca(2+) signals, whereas elevation of the cytosolic Ca(2+) concentration caused a rapid activation of SLAC1 and SLAH3 anion channels. Our data show that the majority of Ca(2+) signals are evoked during the acceleration phase of stomatal closure, which is initiated by OST1. These Ca(2+) signals are likely to activate Ca(2+) -dependent protein kinases, which enhance the activity of S-type anion channels and boost stomatal closure.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Huang, S. and Waadt, R. and Nuhkat, M. and Kollist, H. and Hedrich, R. and Roelfsema, M. R. G.}, biburl = {https://www.bibsonomy.org/bibtex/2d0af80aa479e4fe2bba82cb74e68cc6f/jvsi_all}, doi = {10.1111/nph.15985}, interhash = {56b2096c7c01f9c0ec5376f884d2ca02}, intrahash = {d0af80aa479e4fe2bba82cb74e68cc6f}, issn = {1469-8137 (Electronic) 0028-646X (Print) 0028-646X (Linking)}, journal = {New Phytol}, keywords = {Abscisic Acid/*pharmacology myOwn}, note = {Huang, Shouguang Waadt, Rainer Nuhkat, Maris Kollist, Hannes Hedrich, Rainer Roelfsema, M Rob G eng Research Support, Non-U.S. Gov't England 2019/06/11 New Phytol. 2019 Oct;224(1):177-187. doi: 10.1111/nph.15985. Epub 2019 Jul 19.}, number = 1, pages = {177-187}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {Calcium signals in guard cells enhance the efficiency by which abscisic acid triggers stomatal closure}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/31179540}, volume = 224, year = 2019 }