%0 Journal Article %1 Martnez2013 %A Martínez, Ignacio A. %A Campoy, Susana %A Tort, Meritxell %A Llagostera, Montserrat %A Petrov, Dmitri %D 2013 %I Public Library of Science %J PLoS ONE %K bacteria motility %N 4 %P e61630 %R 10.1371/journal.pone.0061630 %T A Simple Technique Based on a Single Optical Trap for the Determination of Bacterial Swimming Pattern %U http://dx.doi.org/10.1371%2Fjournal.pone.0061630 %V 8 %X Bacterial motility is associated to a wide range of biological processes and it plays a key role in the virulence of many pathogens. Here we describe a method to distinguish the dynamic properties of bacteria by analyzing the statistical functions derived from the trajectories of a bacterium trapped by a single optical beam. The approach is based on the model of the rotation of a solid optically trapped sphere. The technique is easily implemented in a biological laboratory, since with only a small number of optical and electronic components a simple biological microscope can be converted into the required analyzer. To illustrate the functionality of this method, we probed several <inline-formula><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="info:doi/10.1371/journal.pone.0061630.e001" xlink:type="simple"/></inline-formula> <inline-formula><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="info:doi/10.1371/journal.pone.0061630.e002" xlink:type="simple"/></inline-formula> serovar Typhimurium mutants that differed from the wild-type with respect to their swimming patterns. In a further application, the motility dynamics of the <inline-formula><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="info:doi/10.1371/journal.pone.0061630.e003" xlink:type="simple"/></inline-formula> Typhimurium <inline-formula><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="info:doi/10.1371/journal.pone.0061630.e004" xlink:type="simple"/></inline-formula> mutant were characterized.

@article{Martnez2013, abstract = {Bacterial motility is associated to a wide range of biological processes and it plays a key role in the virulence of many pathogens. Here we describe a method to distinguish the dynamic properties of bacteria by analyzing the statistical functions derived from the trajectories of a bacterium trapped by a single optical beam. The approach is based on the model of the rotation of a solid optically trapped sphere. The technique is easily implemented in a biological laboratory, since with only a small number of optical and electronic components a simple biological microscope can be converted into the required analyzer. To illustrate the functionality of this method, we probed several <inline-formula><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="info:doi/10.1371/journal.pone.0061630.e001" xlink:type="simple"/></inline-formula> <inline-formula><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="info:doi/10.1371/journal.pone.0061630.e002" xlink:type="simple"/></inline-formula> serovar Typhimurium mutants that differed from the wild-type with respect to their swimming patterns. In a further application, the motility dynamics of the <inline-formula><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="info:doi/10.1371/journal.pone.0061630.e003" xlink:type="simple"/></inline-formula> Typhimurium <inline-formula><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="info:doi/10.1371/journal.pone.0061630.e004" xlink:type="simple"/></inline-formula> mutant were characterized.}, added-at = {2013-07-09T16:49:53.000+0200}, author = {Martínez, Ignacio A. and Campoy, Susana and Tort, Meritxell and Llagostera, Montserrat and Petrov, Dmitri}, biburl = {https://www.bibsonomy.org/bibtex/254030c4c198d2381763a4289e3badd57/quantentunnel}, doi = {10.1371/journal.pone.0061630}, interhash = {57bafca2bddcd0513cc0ad1f3f5806d4}, intrahash = {54030c4c198d2381763a4289e3badd57}, journal = {PLoS ONE}, keywords = {bacteria motility}, month = {04}, number = 4, pages = {e61630}, publisher = {Public Library of Science}, timestamp = {2013-07-09T16:49:53.000+0200}, title = {A Simple Technique Based on a Single Optical Trap for the Determination of Bacterial Swimming Pattern}, url = {http://dx.doi.org/10.1371%2Fjournal.pone.0061630}, volume = 8, year = 2013 }