%0 Journal Article %1 ALKHATEEB201755 %A Alkhateeb, Rabeaa S. %A Rückert, Christian %A Rupp, Oliver %A Pucker, Boas %A Hublik, Gerd %A Wibberg, Daniel %A Niehaus, Karsten %A Pühler, Alfred %A Vorhölter, Frank-Jörg %D 2017 %J Journal of Biotechnology %K myown %P 55 - 61 %R https://doi.org/10.1016/j.jbiotec.2017.05.009 %T Refined annotation of the complete genome of the phytopathogenic and xanthan producing Xanthomonas campestris pv. campestris strain B100 based on RNA sequence data %U http://www.sciencedirect.com/science/article/pii/S0168165617302407 %V 253 %X Bioinformatics tools and gene expression data were applied to identify new genes and to enhance the accuracy in genomic feature predictions for Xanthomonas campestris pv. campestris (Xcc) B100, a pathogen of cruciferous plants and model strain for the biosynthesis of xanthan, a polysaccharide with a multitude of commercial applications as a thickening agent. Results from 5′-enriched end RNA sequencing (RNA-seq) and total transcriptome RNA-seq experiments were used for this purpose. Functional gene annotations were updated where new evidence had emerged and start codon predictions were enhanced for 153 protein-coding genes (CDS). In total, 32 novel CDS, and 176 novel RNA genes and features were predicted, among them 77 isogenes of the small non-coding RNA sX9. Furthermore, the RNA-seq data facilitated the identification of 848 operons that included a total of 2551 CDS besides 1667 CDS that were mono-cistronically expressed.

@article{ALKHATEEB201755, abstract = {Bioinformatics tools and gene expression data were applied to identify new genes and to enhance the accuracy in genomic feature predictions for Xanthomonas campestris pv. campestris (Xcc) B100, a pathogen of cruciferous plants and model strain for the biosynthesis of xanthan, a polysaccharide with a multitude of commercial applications as a thickening agent. Results from 5′-enriched end RNA sequencing (RNA-seq) and total transcriptome RNA-seq experiments were used for this purpose. Functional gene annotations were updated where new evidence had emerged and start codon predictions were enhanced for 153 protein-coding genes (CDS). In total, 32 novel CDS, and 176 novel RNA genes and features were predicted, among them 77 isogenes of the small non-coding RNA sX9. Furthermore, the RNA-seq data facilitated the identification of 848 operons that included a total of 2551 CDS besides 1667 CDS that were mono-cistronically expressed.}, added-at = {2018-11-08T06:41:05.000+0100}, author = {Alkhateeb, Rabeaa S. and Rückert, Christian and Rupp, Oliver and Pucker, Boas and Hublik, Gerd and Wibberg, Daniel and Niehaus, Karsten and Pühler, Alfred and Vorhölter, Frank-Jörg}, biburl = {https://www.bibsonomy.org/bibtex/2e2638143845920259c2af72ba42f31e8/bpucker}, description = {Refined annotation of the complete genome of the phytopathogenic and xanthan producing Xanthomonas campestris pv. campestris strain B100 based on RNA sequence data - ScienceDirect}, doi = {https://doi.org/10.1016/j.jbiotec.2017.05.009}, interhash = {5844b6ea596938a5251eb834d56713f9}, intrahash = {e2638143845920259c2af72ba42f31e8}, issn = {0168-1656}, journal = {Journal of Biotechnology}, keywords = {myown}, pages = {55 - 61}, timestamp = {2018-11-08T06:41:05.000+0100}, title = {Refined annotation of the complete genome of the phytopathogenic and xanthan producing Xanthomonas campestris pv. campestris strain B100 based on RNA sequence data}, url = {http://www.sciencedirect.com/science/article/pii/S0168165617302407}, volume = 253, year = 2017 }