%0 Journal Article %1 elsayed2018metabolomic %A Elsayed, Y. %A Refaat, J. %A Abdelmohsen, U. R. %A Othman, E. M. %A Stopper, H. %A Fouad, M. A. %D 2018 %J Phytochem Anal %K Animals myOwn %N 6 %P 543-548 %R 10.1002/pca.2765 %T Metabolomic profiling and biological investigation of the marine sponge-derived bacterium Rhodococcus sp. UA13 %U https://www.ncbi.nlm.nih.gov/pubmed/29672972 %V 29 %X INTRODUCTION: Marine sponge-associated actinomycetes are potent sources of bioactive natural products of pharmaceutical significance. They also contributed to the discovery of several clinically relevant antimicrobials. OBJECTIVE: To apply the non-targeted metabolomics approach in chemical profiling of the sponge-derived bacterium Rhodococcus sp. UA13, formerly recovered from the Red Sea sponge Callyspongia aff. Implexa, along with testing for the anti-infective potential of its different fractions. METHODOLOGY: Metabolomic analysis of the crude extract was carried out using liquid chromatography with high resolution electrospray ionisation mass spectrometry (LC-HR-ESI-MS) for dereplication purposes. Besides, the three major fractions (ethyl acetate, methanol, and n-butanol) obtained by chromatographic fractionation of the crude extract were evaluated for their anti-infective properties. RESULTS: A variety of metabolites, mostly peptides, were characterised herein for the first time from the genus Rhodococcus. Among the tested samples, the n-butanol fraction showed potent inhibitory activities against Staphylococcus aureus, Candida albicans, and Trypanosoma brucei brucei with IC(50) values of 9.3, 6.7, and 8.7 mug/mL, respectively, whereas only the ethyl acetate fraction was active against Chlamydia trachomatis (IC(50) = 18.9 mug/mL). In contrast, both fractions did not exert anti-infective actions against Enterococcus faecalis and Leishmania major, whereas the methanol fraction was totally inactive against all the tested organisms. CONCLUSION: This study showed the helpfulness of the established procedure in metabolic profiling of marine actinomycetes using liquid chromatography mass spectrometry (LC-MS) data, which aids in reducing the complex isolation steps during their chemical characterisation. The anti-infective spectrum of their metabolites is also interestingly relevant to future drug development.

@article{elsayed2018metabolomic, abstract = {INTRODUCTION: Marine sponge-associated actinomycetes are potent sources of bioactive natural products of pharmaceutical significance. They also contributed to the discovery of several clinically relevant antimicrobials. OBJECTIVE: To apply the non-targeted metabolomics approach in chemical profiling of the sponge-derived bacterium Rhodococcus sp. UA13, formerly recovered from the Red Sea sponge Callyspongia aff. Implexa, along with testing for the anti-infective potential of its different fractions. METHODOLOGY: Metabolomic analysis of the crude extract was carried out using liquid chromatography with high resolution electrospray ionisation mass spectrometry (LC-HR-ESI-MS) for dereplication purposes. Besides, the three major fractions (ethyl acetate, methanol, and n-butanol) obtained by chromatographic fractionation of the crude extract were evaluated for their anti-infective properties. RESULTS: A variety of metabolites, mostly peptides, were characterised herein for the first time from the genus Rhodococcus. Among the tested samples, the n-butanol fraction showed potent inhibitory activities against Staphylococcus aureus, Candida albicans, and Trypanosoma brucei brucei with IC(50) values of 9.3, 6.7, and 8.7 mug/mL, respectively, whereas only the ethyl acetate fraction was active against Chlamydia trachomatis (IC(50) = 18.9 mug/mL). In contrast, both fractions did not exert anti-infective actions against Enterococcus faecalis and Leishmania major, whereas the methanol fraction was totally inactive against all the tested organisms. CONCLUSION: This study showed the helpfulness of the established procedure in metabolic profiling of marine actinomycetes using liquid chromatography mass spectrometry (LC-MS) data, which aids in reducing the complex isolation steps during their chemical characterisation. The anti-infective spectrum of their metabolites is also interestingly relevant to future drug development.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Elsayed, Y. and Refaat, J. and Abdelmohsen, U. R. and Othman, E. M. and Stopper, H. and Fouad, M. A.}, biburl = {https://www.bibsonomy.org/bibtex/237ecf4a2e3e3b04ac3e7c3e7d81990a2/jvsi_all}, doi = {10.1002/pca.2765}, interhash = {5b9620764657621e4d54d4d4302ef712}, intrahash = {37ecf4a2e3e3b04ac3e7c3e7d81990a2}, issn = {1099-1565 (Electronic) 0958-0344 (Linking)}, journal = {Phytochem Anal}, keywords = {Animals myOwn}, note = {Elsayed, Yasmin Refaat, John Abdelmohsen, Usama Ramadan Othman, Eman Maher Stopper, Helga Fouad, Mostafa Ahmed eng England 2018/04/20 Phytochem Anal. 2018 Nov;29(6):543-548. doi: 10.1002/pca.2765. Epub 2018 Apr 19.}, number = 6, pages = {543-548}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {Metabolomic profiling and biological investigation of the marine sponge-derived bacterium Rhodococcus sp. UA13}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/29672972}, volume = 29, year = 2018 }