Article,
Effects of a Mangifera indica L. stem bark extract and mangiferin on the radiation-induced DNA damage in human lymphocytes and lymphoblastic cells
Cell Proliferation, (February 2014)
Abstract
Objectives: Mangifera indica L. (mango) stem bark aqueous extract (MSBE) that has antioxidant, antiinflammatory and immunomodulatory properties, can be obtained in Cuba. It is rich in polyphenols,
where mangiferin is the main component. In this study, we have tested DNA damage and protection
effects of MSBE and mangiferin on primary human lymphocytes and lymphoblastoid cells.
Material and methods: Cell suspensions were incubated with the products (50–1000 ug/ml) for experiments
on damage induction, and evaluation of any potential protective effects (5–100 ug/ml) for 60 min at 37 °C. Irradiation was performed using a g-ray source, absorbed dose 5 Gy. At the end of exposure, DNA damage, protection and repair processes were evaluated using the comet assay.
Results: MSBE (100–1000 ug/ml) induced DNA damage in a concentration dependent manner in both cell types tested, primary cells being more sensitive. Mangiferin (200 ug/ml) only induced light DNA damage at higher concentrations. DNA repair capacity was not affected after MSBE or mangiferin exposure. On the other hand, MSBE (25 and 50 ug/ml) and mangiferin (5–25 ug/ml)protected against gamma radiation-induced DNA damage.
Conclusions: These results show MSBE has protector or harmful effects on DNA in vitro depending on the experimental conditions, which suggest that the extract could be acting as an antioxidant or pro-oxidant product. Mangiferin was involved in protective effects of the extract.
