%0 Journal Article %1 mickShy1CouplesCox12007 %A Mick, David U. %A Wagner, Karina %A van der Laan, Martin %A Frazier, Ann E. %A Perschil, Inge %A Pawlas, Magdalena %A Meyer, Helmut E. %A Warscheid, Bettina %A Rehling, Peter %C England %D 2007 %J The EMBO journal %K *Gene Binding,Protein Biological,Protein Biosynthesis,DNA Complex Expression Fungal,*Protein Fungal,Membrane IV/*metabolism,Genes Interaction Mapping,Saccharomyces Mitochondrial/metabolism,Electron Proteins/*physiology,Saccharomyces Proteins/metabolism,Models Proteins/metabolism/*physiology,Mitochondria/metabolism,Mitochondrial Regulation Transport Transport,Electron cerevisiae cerevisiae/*metabolism,to_read %N 20 %P 4347--4358 %R 10.1038/sj.emboj.7601862 %T Shy1 Couples Cox1 Translational Regulation to Cytochrome c Oxidase Assembly. %V 26 %X Cytochrome c oxidase (complex IV) of the respiratory chain is assembled from nuclear and mitochondrially-encoded subunits. Defects in the assembly process lead to severe human disorders such as Leigh syndrome. Shy1 is an assembly factor for complex IV in Saccharomyces cerevisiae and mutations of its human homolog, SURF1, are the most frequent cause for Leigh syndrome. We report that Shy1 promotes complex IV biogenesis through association with different protein modules; Shy1 interacts with Mss51 and Cox14, translational regulators of Cox1. Additionally, Shy1 associates with the subcomplexes of complex IV that are potential assembly intermediates. Formation of these subcomplexes depends on Coa1 (YIL157c), a novel assembly factor that cooperates with Shy1. Moreover, partially assembled forms of complex IV bound to Shy1 and Cox14 can associate with the bc1 complex to form transitional supercomplexes. We suggest that Shy1 links Cox1 translational regulation to complex IV assembly and supercomplex formation.

@article{mickShy1CouplesCox12007, abstract = {Cytochrome c oxidase (complex IV) of the respiratory chain is assembled from nuclear and mitochondrially-encoded subunits. Defects in the assembly process lead to severe human disorders such as Leigh syndrome. Shy1 is an assembly factor for complex IV in Saccharomyces cerevisiae and mutations of its human homolog, SURF1, are the most frequent cause for Leigh syndrome. We report that Shy1 promotes complex IV biogenesis through association with different protein modules; Shy1 interacts with Mss51 and Cox14, translational regulators of Cox1. Additionally, Shy1 associates with the subcomplexes of complex IV that are potential assembly intermediates. Formation of these subcomplexes depends on Coa1 (YIL157c), a novel assembly factor that cooperates with Shy1. Moreover, partially assembled forms of complex IV bound to Shy1 and Cox14 can associate with the bc1 complex to form transitional supercomplexes. We suggest that Shy1 links Cox1 translational regulation to complex IV assembly and supercomplex formation.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {England}, author = {Mick, David U. and Wagner, Karina and {van der Laan}, Martin and Frazier, Ann E. and Perschil, Inge and Pawlas, Magdalena and Meyer, Helmut E. and Warscheid, Bettina and Rehling, Peter}, biburl = {https://www.bibsonomy.org/bibtex/26319658a0d0596ee5b9b1c0ed196bc45/warscheidlab}, doi = {10.1038/sj.emboj.7601862}, interhash = {665da083eb2a87d6ade8e1828de8a7eb}, intrahash = {6319658a0d0596ee5b9b1c0ed196bc45}, issn = {1460-2075 0261-4189}, journal = {The EMBO journal}, keywords = {*Gene Binding,Protein Biological,Protein Biosynthesis,DNA Complex Expression Fungal,*Protein Fungal,Membrane IV/*metabolism,Genes Interaction Mapping,Saccharomyces Mitochondrial/metabolism,Electron Proteins/*physiology,Saccharomyces Proteins/metabolism,Models Proteins/metabolism/*physiology,Mitochondria/metabolism,Mitochondrial Regulation Transport Transport,Electron cerevisiae cerevisiae/*metabolism,to_read}, langid = {english}, month = oct, number = 20, pages = {4347--4358}, pmcid = {PMC2034671}, pmid = {17882259}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {Shy1 Couples {{Cox1}} Translational Regulation to Cytochrome c Oxidase Assembly.}, volume = 26, year = 2007 }