%0 Journal Article %1 Liu2015Genome %A Liu, Rongming %A Bassalo, Marcelo C. %A Zeitoun, Ramsey I. %A Gill, Ryan T. %D 2015 %J Metabolic engineering %K genome-scale metabolic-engineering %P 143--154 %R 10.1016/j.ymben.2015.09.013 %T Genome scale engineering techniques for metabolic engineering. %U http://dx.doi.org/10.1016/j.ymben.2015.09.013 %V 32 %X Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in genome-scale engineering technologies. Metabolic engineering has been generally defined by the use of iterative cycles of rational genome modifications, strain analysis and characterization, and a synthesis step that fuels additional hypothesis generation. This cycle mirrors the Design-Build-Test-Learn cycle followed throughout various engineering fields that has recently become a defining aspect of synthetic biology. This review will attempt to summarize recent genome-scale design, build, test, and learn technologies and relate their use to a range of metabolic engineering applications. Copyright \copyright 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

@article{Liu2015Genome, abstract = {Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in genome-scale engineering technologies. Metabolic engineering has been generally defined by the use of iterative cycles of rational genome modifications, strain analysis and characterization, and a synthesis step that fuels additional hypothesis generation. This cycle mirrors the {Design-Build}-{Test-Learn} cycle followed throughout various engineering fields that has recently become a defining aspect of synthetic biology. This review will attempt to summarize recent genome-scale design, build, test, and learn technologies and relate their use to a range of metabolic engineering applications. Copyright {\copyright} 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Liu, Rongming and Bassalo, Marcelo C. and Zeitoun, Ramsey I. and Gill, Ryan T.}, biburl = {https://www.bibsonomy.org/bibtex/2f9934675cd84c2a4d672ef1a0f300e61/karthikraman}, citeulike-article-id = {13801591}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ymben.2015.09.013}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/26453944}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=26453944}, day = 11, doi = {10.1016/j.ymben.2015.09.013}, interhash = {686ea34db9691a242767962d3dd11602}, intrahash = {f9934675cd84c2a4d672ef1a0f300e61}, issn = {1096-7184}, journal = {Metabolic engineering}, keywords = {genome-scale metabolic-engineering}, month = oct, pages = {143--154}, pmid = {26453944}, posted-at = {2015-11-04 06:24:06}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Genome scale engineering techniques for metabolic engineering.}, url = {http://dx.doi.org/10.1016/j.ymben.2015.09.013}, volume = 32, year = 2015 }