%0 Journal Article %1 mei2010combinatorial %A Mei, Ying %A Saha, Krishanu %A Bogatyrev, Said R. %A Yang, Jing %A Hook, Andrew L. %A Kalcioglu, Z. Ilke %A Cho, Seung-Woo %A Mitalipova, Maisam %A Pyzocha, Neena %A Rojas, Fredrick %A Van Vliet, Krystyn J. %A Davies, Martyn C. %A Alexander, Morgan R. %A Langer, Robert %A Jaenisch, Rudolf %A Anderson, Daniel G. %D 2010 %I Nature Publishing Group %J Nat Mater %K biomaterial microarray nextgen screening stemcell %N 9 %P 768--778 %T Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells %U http://dx.doi.org/10.1038/nmat2812 %V 9 %X Both human embryonic stem cells and induced pluripotent stem cells can self-renew indefinitely in culture; however, present methods to clonally grow them are inefficient and poorly defined for genetic manipulation and therapeutic purposes. Here we develop the first chemically defined, xeno-free, feeder-free synthetic substrates to support robust self-renewal of fully dissociated human embryonic stem and induced pluripotent stem cells. Material properties including wettability, surface topography, surface chemistry and indentation elastic modulus of all polymeric substrates were quantified using high-throughput methods to develop structure–function relationships between material properties and biological performance. These analyses show that optimal human embryonic stem cell substrates are generated from monomers with high <span class="highlight-compound highlight-off annotation" data-related-id=ännotation-compound-100001">acrylate</span> content, have a moderate wettability and employ integrin <i>α</i>_v<i>β</i>₃ and <i>α</i>_v<i>β</i>₅ engagement with adsorbed vitronectin to promote colony formation. The structure–function methodology employed herein provides a general framework for the combinatorial development of synthetic substrates for stem cell culture.</p> <p class="full-text-link right-arrow"><a href="/nmat/journal/v9/n9/full/nmat2812.html">View full text</a>

@article{mei2010combinatorial, abstract = {Both human embryonic stem cells and induced pluripotent stem cells can self-renew indefinitely in culture; however, present methods to clonally grow them are inefficient and poorly defined for genetic manipulation and therapeutic purposes. Here we develop the first chemically defined, xeno-free, feeder-free synthetic substrates to support robust self-renewal of fully dissociated human embryonic stem and induced pluripotent stem cells. Material properties including wettability, surface topography, surface chemistry and indentation elastic modulus of all polymeric substrates were quantified using high-throughput methods to develop structure–function relationships between material properties and biological performance. These analyses show that optimal human embryonic stem cell substrates are generated from monomers with high <span class="highlight-compound highlight-off annotation" data-related-id="annotation-compound-100001">acrylate</span> content, have a moderate wettability and employ integrin <i>α</i>_v<i>β</i>₃ and <i>α</i>_v<i>β</i>₅ engagement with adsorbed vitronectin to promote colony formation. The structure–function methodology employed herein provides a general framework for the combinatorial development of synthetic substrates for stem cell culture.</p> <p class="full-text-link right-arrow"><a href="/nmat/journal/v9/n9/full/nmat2812.html">View full text</a>}, added-at = {2016-02-15T10:35:09.000+0100}, author = {Mei, Ying and Saha, Krishanu and Bogatyrev, Said R. and Yang, Jing and Hook, Andrew L. and Kalcioglu, Z. Ilke and Cho, Seung-Woo and Mitalipova, Maisam and Pyzocha, Neena and Rojas, Fredrick and Van Vliet, Krystyn J. and Davies, Martyn C. and Alexander, Morgan R. and Langer, Robert and Jaenisch, Rudolf and Anderson, Daniel G.}, biburl = {https://www.bibsonomy.org/bibtex/2443bc9aa1c0847449235737934299ab4/bkoch}, comment = {10.1038/nmat2812}, description = {Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells : Nature Materials : Nature Publishing Group}, interhash = {6f19e5f821deff5b4fe071dbfde1a49c}, intrahash = {443bc9aa1c0847449235737934299ab4}, issn = {14761122}, journal = {Nat Mater}, keywords = {biomaterial microarray nextgen screening stemcell}, month = sep, number = 9, pages = {768--778}, publisher = {Nature Publishing Group}, timestamp = {2016-02-15T10:35:09.000+0100}, title = {Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells}, url = {http://dx.doi.org/10.1038/nmat2812}, volume = 9, year = 2010 }