Luminescence Standards for Macro- and Microspectrofluorometry
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chapter 8, page 98-126. (1989)

Requirements for standards used in macro- and microspectrofluorometry differ, depending on whether they are used for instrument calibration, standardization, or assessment of method accuracy. Specific examples are given of standards for quantum yield, number of quanta, and decay time, and for calibration of instrument parameters, including wavelength, spectral responsivity (determining correction factors for luminescence spectra), stability, and linearity. Differences in requirements for macro- and micro-standards are considered, and specific materials used for each are compared. Pure compounds and matrix-matched standards are listed for standardization and assessment of method accuracy, and existing Standard Reference Materials are discussed.
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