Abstract
The present study focuses on apatite coating on collagen films, with
various different densities of carboxyl groups, using an alternate
soaking process. Anionic collagen (AC), which has different densities of
carboxylic groups compared to native collagen (NC), was obtained by
hydrolysis of carboxyamides of asparagine and glutamine residues. From
X-ray diffraction analysis, apatite was found to be coated on AC and NC
films. Peaks ascribed to apatite were observed at 26 degrees and 32
degrees in the diffraction patterns of hydroxyapatite crystals. The
amount of apatite coated on both AC and NC collagen films continued to
increase up to 100 reaction cycles. However, there is a significant
difference in apatite coating between the two films. The amount of
apatite formed on the surface of AC film increased 1.24 times faster
than on NC film. The scanning electron photomicrograph images of the
mineralized NC and the AC film coatings formed after 100 cycles show
that regular porous apatite coating had formed within the collagen
fibrils. These results suggest that the higher content of carboxyl
groups in AC plays an effective role in the heterogeneous nucleation of
apatite in the body environment. (c) 2007 Acta Materialia Inc. Published
by Elsevier Ltd. All rights reserved.
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