%0 Journal Article %1 WOS:000249625300017 %A Goes, J C %A Figueiro, S D %A Oliveira, A M %A Macedo, A A M %A Silva, C C %A Ricardo, N M P S %A Sombra, A S B %C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND %D 2007 %I ELSEVIER SCI LTD %J ACTA BIOMATERIALIA %K alternate anionic coating; collagen film; process} soaking {apatite %N 5 %P 773-778 %R 10.1016/j.actbio.2007.02.008 %T Apatite coating on anionic and native collagen films by an alternate soaking process %V 3 %X The present study focuses on apatite coating on collagen films, with various different densities of carboxyl groups, using an alternate soaking process. Anionic collagen (AC), which has different densities of carboxylic groups compared to native collagen (NC), was obtained by hydrolysis of carboxyamides of asparagine and glutamine residues. From X-ray diffraction analysis, apatite was found to be coated on AC and NC films. Peaks ascribed to apatite were observed at 26 degrees and 32 degrees in the diffraction patterns of hydroxyapatite crystals. The amount of apatite coated on both AC and NC collagen films continued to increase up to 100 reaction cycles. However, there is a significant difference in apatite coating between the two films. The amount of apatite formed on the surface of AC film increased 1.24 times faster than on NC film. The scanning electron photomicrograph images of the mineralized NC and the AC film coatings formed after 100 cycles show that regular porous apatite coating had formed within the collagen fibrils. These results suggest that the higher content of carboxyl groups in AC plays an effective role in the heterogeneous nucleation of apatite in the body environment. (c) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

@article{WOS:000249625300017, abstract = {The present study focuses on apatite coating on collagen films, with various different densities of carboxyl groups, using an alternate soaking process. Anionic collagen (AC), which has different densities of carboxylic groups compared to native collagen (NC), was obtained by hydrolysis of carboxyamides of asparagine and glutamine residues. From X-ray diffraction analysis, apatite was found to be coated on AC and NC films. Peaks ascribed to apatite were observed at 26 degrees and 32 degrees in the diffraction patterns of hydroxyapatite crystals. The amount of apatite coated on both AC and NC collagen films continued to increase up to 100 reaction cycles. However, there is a significant difference in apatite coating between the two films. The amount of apatite formed on the surface of AC film increased 1.24 times faster than on NC film. The scanning electron photomicrograph images of the mineralized NC and the AC film coatings formed after 100 cycles show that regular porous apatite coating had formed within the collagen fibrils. These results suggest that the higher content of carboxyl groups in AC plays an effective role in the heterogeneous nucleation of apatite in the body environment. (c) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.}, added-at = {2022-05-23T20:00:14.000+0200}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND}, author = {Goes, J C and Figueiro, S D and Oliveira, A M and Macedo, A A M and Silva, C C and Ricardo, N M P S and Sombra, A S B}, biburl = {https://www.bibsonomy.org/bibtex/2434dea1a918d57e07d2b2fd2ba278676/ppgfis_ufc_br}, doi = {10.1016/j.actbio.2007.02.008}, interhash = {7288b2679e7ef555761b70e9c32f309d}, intrahash = {434dea1a918d57e07d2b2fd2ba278676}, issn = {1742-7061}, journal = {ACTA BIOMATERIALIA}, keywords = {alternate anionic coating; collagen film; process} soaking {apatite}, number = 5, pages = {773-778}, publisher = {ELSEVIER SCI LTD}, pubstate = {published}, timestamp = {2022-05-23T20:00:14.000+0200}, title = {Apatite coating on anionic and native collagen films by an alternate soaking process}, tppubtype = {article}, volume = 3, year = 2007 }