%0 Journal Article %1 Crocker1996 %A Crocker, John C. %A Grier, David G. %D 1996 %J Journal of Colloid and Interface Science %K imageprocessing tracking %N 1 %P 298 - 310 %R 10.1006/jcis.1996.0217 %T Methods of Digital Video Microscopy for Colloidal Studies %U http://www.sciencedirect.com/science/article/pii/S0021979796902179 %V 179 %X We describe a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions. In a typical application, these direct imaging techniques can locate submicrometer spheres to within 10 nm in the focal plane and 150 nm in depth. Combining information from a sequence of video images into single-particle trajectories makes possible measurements of quantities of fundamental and practical interest such as diffusion coefficients and pair-wise interaction potentials. The measurements we describe in detail combine the outstanding resolution of digital imaging with video-synchronized optical trapping to obtain highly accurate and reproducible results very rapidly.

@article{Crocker1996, abstract = {We describe a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions. In a typical application, these direct imaging techniques can locate submicrometer spheres to within 10 nm in the focal plane and 150 nm in depth. Combining information from a sequence of video images into single-particle trajectories makes possible measurements of quantities of fundamental and practical interest such as diffusion coefficients and pair-wise interaction potentials. The measurements we describe in detail combine the outstanding resolution of digital imaging with video-synchronized optical trapping to obtain highly accurate and reproducible results very rapidly. }, added-at = {2013-10-01T09:35:54.000+0200}, author = {Crocker, John C. and Grier, David G.}, biburl = {https://www.bibsonomy.org/bibtex/2ea0a9757ec416a35b6cbca253d502c31/quantentunnel}, description = {tracking algorithm}, doi = {10.1006/jcis.1996.0217}, interhash = {73435f8a6f000a12adcfa7d2f1e236a7}, intrahash = {ea0a9757ec416a35b6cbca253d502c31}, issn = {0021-9797}, journal = {Journal of Colloid and Interface Science }, keywords = {imageprocessing tracking}, number = 1, pages = {298 - 310}, timestamp = {2013-10-01T09:35:54.000+0200}, title = {Methods of Digital Video Microscopy for Colloidal Studies }, url = {http://www.sciencedirect.com/science/article/pii/S0021979796902179}, volume = 179, year = 1996 }