%0 Journal Article %1 nuhkat2021rapid %A Nuhkat, M. %A Brosche, M. %A Stoelzle-Feix, S. %A Dietrich, P. %A Hedrich, R. %A Roelfsema, M. R. G. %A Kollist, H. %D 2021 %J New Phytol %K Calcium myOwn %N 4 %P 1692-1702 %R 10.1111/nph.17711 %T Rapid depolarization and cytosolic calcium increase go hand-in-hand in mesophyll cells' ozone response %U https://www.ncbi.nlm.nih.gov/pubmed/34482538 %V 232 %X Plant stress signalling involves bursts of reactive oxygen species (ROS), which can be mimicked by the application of acute pulses of ozone. Such ozone-pulses inhibit photosynthesis and trigger stomatal closure in a few minutes, but the signalling that underlies these responses remains largely unknown. We measured changes in Arabidopsis thaliana gas exchange after treatment with acute pulses of ozone and set up a system for simultaneous measurement of membrane potential and cytosolic calcium with the fluorescent reporter R-GECO1. We show that within 1 min, prior to stomatal closure, O(3) triggered a drop in whole-plant CO(2) uptake. Within this early phase, O(3) pulses (200-1000 ppb) elicited simultaneous membrane depolarization and cytosolic calcium increase, whereas these pulses had no long-term effect on either stomatal conductance or photosynthesis. In contrast, pulses of 5000 ppb O(3) induced cell death, systemic Ca(2+) signals and an irreversible drop in stomatal conductance and photosynthetic capacity. We conclude that mesophyll cells respond to ozone in a few seconds by distinct pattern of plasma membrane depolarizations accompanied by an increase in the cytosolic calcium ion (Ca(2+) ) level. These responses became systemic only at very high ozone concentrations. Thus, plants have rapid mechanism to sense and discriminate the strength of ozone signals.

@article{nuhkat2021rapid, abstract = {Plant stress signalling involves bursts of reactive oxygen species (ROS), which can be mimicked by the application of acute pulses of ozone. Such ozone-pulses inhibit photosynthesis and trigger stomatal closure in a few minutes, but the signalling that underlies these responses remains largely unknown. We measured changes in Arabidopsis thaliana gas exchange after treatment with acute pulses of ozone and set up a system for simultaneous measurement of membrane potential and cytosolic calcium with the fluorescent reporter R-GECO1. We show that within 1 min, prior to stomatal closure, O(3) triggered a drop in whole-plant CO(2) uptake. Within this early phase, O(3) pulses (200-1000 ppb) elicited simultaneous membrane depolarization and cytosolic calcium increase, whereas these pulses had no long-term effect on either stomatal conductance or photosynthesis. In contrast, pulses of 5000 ppb O(3) induced cell death, systemic Ca(2+) signals and an irreversible drop in stomatal conductance and photosynthetic capacity. We conclude that mesophyll cells respond to ozone in a few seconds by distinct pattern of plasma membrane depolarizations accompanied by an increase in the cytosolic calcium ion (Ca(2+) ) level. These responses became systemic only at very high ozone concentrations. Thus, plants have rapid mechanism to sense and discriminate the strength of ozone signals.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Nuhkat, M. and Brosche, M. and Stoelzle-Feix, S. and Dietrich, P. and Hedrich, R. and Roelfsema, M. R. G. and Kollist, H.}, biburl = {https://www.bibsonomy.org/bibtex/22f380be5d2ad171e71a3b6df27635afa/jvsi_all}, doi = {10.1111/nph.17711}, interhash = {7385014d324b4c2b17d7d1cf1dc175c7}, intrahash = {2f380be5d2ad171e71a3b6df27635afa}, issn = {1469-8137 (Electronic) 0028-646X (Linking)}, journal = {New Phytol}, keywords = {Calcium myOwn}, note = {Nuhkat, Maris Brosche, Mikael Stoelzle-Feix, Sonja Dietrich, Petra Hedrich, Rainer Roelfsema, M Rob G Kollist, Hannes eng Research Support, Non-U.S. Gov't England 2021/09/06 New Phytol. 2021 Nov;232(4):1692-1702. doi: 10.1111/nph.17711. Epub 2021 Sep 23.}, number = 4, pages = {1692-1702}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {Rapid depolarization and cytosolic calcium increase go hand-in-hand in mesophyll cells' ozone response}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/34482538}, volume = 232, year = 2021 }