%0 Journal Article %1 Lehn_1998_220 %A Lehnart, S. E. %A Schillinger, W. %A Pieske, B. %A Prestle, J. %A Just, H. %A Hasenfuss, G. %D 1998 %J Ann. N. Y. Acad. Sci. %K , 10603950 ATPase, Animals, Calcium Calcium, Calcium-Binding Calreticulin, Calsequestrin, Cardiomyopathy, Channel, Congestive, Contraction, Dilated, Exchanger, Failure, Heart Humans, Myocardial Proteins, Receptor Release Reticulum, Ribonucleoproteins, Ryanodine Sarcoplasmic Sodium-Calcium {C}a$^{2+}$-Transporting %P 220--230 %T Sarcoplasmic reticulum proteins in heart failure. %U http://www.annalsnyas.org/cgi/content/full/853/1/220 %V 853 %X Altered calcium homeostasis may play a key role in the pathophysiology of human heart failure. Levels of sarcoplasmic reticulum (SR) proteins and sarcolemmal Na$^+$-Ca$^2+$ exchanger were analyzed by Western blot in failing and nonfailing human myocardium and related to myocardial function. Levels of the SR calcium release channel and of calcium storage proteins (calsequestrin and calreticulin) were not different in nonfailing and failing hearts. However, proteins involved in calcium removal were significantly altered in the failing human heart: (1) SR-Ca$^2+$-ATPase levels and the ratio of SR-Ca$^2+$-ATPase to its inhibitory protein phospholamban were significantly decreased, and (2) Na$^+$-Ca$^2+$ exchanger levels and the ratio of Na$^+$-Ca$^2+$ exchanger to SR-Ca$^2+$-ATPase were significantly increased. SR-Ca$^2+$-ATPase levels were closely correlated to systolic function as evaluated by frequency potentiation of contractile force. The frequency-dependent rise of diastolic force was inversely correlated with protein levels of Na$^+$-Ca$^2+$ exchanger. These findings indicate that altered expression of SR-Ca$^2+$-ATPase and Na$^+$-Ca$^2+$ exchanger is relevant for altered systolic and diastolic function in human heart failure.

@article{Lehn_1998_220, abstract = {Altered calcium homeostasis may play a key role in the pathophysiology of human heart failure. Levels of sarcoplasmic reticulum (SR) proteins and sarcolemmal {N}a$^{+}$-{C}a$^{2+}$ exchanger were analyzed by Western blot in failing and nonfailing human myocardium and related to myocardial function. Levels of the SR calcium release channel and of calcium storage proteins (calsequestrin and calreticulin) were not different in nonfailing and failing hearts. However, proteins involved in calcium removal were significantly altered in the failing human heart: (1) SR-{C}a$^{2+}$-ATPase levels and the ratio of SR-{C}a$^{2+}$-ATPase to its inhibitory protein phospholamban were significantly decreased, and (2) {N}a$^{+}$-{C}a$^{2+}$ exchanger levels and the ratio of {N}a$^{+}$-{C}a$^{2+}$ exchanger to SR-{C}a$^{2+}$-ATPase were significantly increased. SR-{C}a$^{2+}$-ATPase levels were closely correlated to systolic function as evaluated by frequency potentiation of contractile force. The frequency-dependent rise of diastolic force was inversely correlated with protein levels of {N}a$^{+}$-{C}a$^{2+}$ exchanger. These findings indicate that altered expression of SR-{C}a$^{2+}$-ATPase and {N}a$^{+}$-{C}a$^{2+}$ exchanger is relevant for altered systolic and diastolic function in human heart failure.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Lehnart, S. E. and Schillinger, W. and Pieske, B. and Prestle, J. and Just, H. and Hasenfuss, G.}, biburl = {https://www.bibsonomy.org/bibtex/2510650970128802b06d056c84d44518e/hake}, description = {The whole bibliography file I use.}, file = {Lehn_1998_220.pdf:Lehn_1998_220.pdf:PDF}, interhash = {7978bc9320d6a13e0e4503dbb3947465}, intrahash = {510650970128802b06d056c84d44518e}, journal = {Ann. N. Y. Acad. Sci.}, key = 114, keywords = {, 10603950 ATPase, Animals, Calcium Calcium, Calcium-Binding Calreticulin, Calsequestrin, Cardiomyopathy, Channel, Congestive, Contraction, Dilated, Exchanger, Failure, Heart Humans, Myocardial Proteins, Receptor Release Reticulum, Ribonucleoproteins, Ryanodine Sarcoplasmic Sodium-Calcium {C}a$^{2+}$-Transporting}, month = Sep, pages = {220--230}, pmid = {10603950}, timestamp = {2009-06-03T11:21:19.000+0200}, title = {Sarcoplasmic reticulum proteins in heart failure.}, url = {http://www.annalsnyas.org/cgi/content/full/853/1/220}, volume = 853, year = 1998 }