%0 Journal Article %1 citeulike:1561293 %A Ishikawa, Masaya %D 1984 %J Plant Physiol. %K anatomy, cellular, citeulikeExport cryo, damage, freezing, grass, ice, intracellular, nucleation, snowgumpapermaybe %N 1 %P 196--202 %T Deep Supercooling in Most Tissues of Wintering Sasa senanensis and Its Mechanism in Leaf Blade Tissues %U http://www.plantphysiol.org/cgi/content/abstract/75/1/196 %V 75 %X Cold hardiness of leaf blades, leaf sheaths, culms, rhizomes, and leaf buds in wintering Sasa senanensis (Fr. et Sav.) Rehder, a dwarf bamboo, was studied paying special attention to the types of resistance mechanisms which were determined with differential thermal analysis. Coincidence of LT25 (lethal temperature at which 25\% of the tissues are injured) with the initiation temperature of LTE (low temperature exotherm) suggested that all of these tissues described above owe their cold hardiness mechanism mostly to deep supercooling. Deep supercooling in leaf blades was also substantiated with microscopic observations, suggesting that the units of supercooling were minute tissues compartmentalized by longitudinal and cross veins. It was also shown that cooling rates and storage of shoots at -5degreesC for 1 to 5 days in the ice-inoculated state did not greatly affect the supercooling ability of leaf blades. Sasa senanensis seemed to exhibit a unique strategy against prolonged subzero temperature, and its leaves would be a good system for the study on mechanisms of deep undercooling in plants.

@article{citeulike:1561293, abstract = {Cold hardiness of leaf blades, leaf sheaths, culms, rhizomes, and leaf buds in wintering Sasa senanensis (Fr. et Sav.) Rehder, a dwarf bamboo, was studied paying special attention to the types of resistance mechanisms which were determined with differential thermal analysis. Coincidence of LT25 (lethal temperature at which 25\% of the tissues are injured) with the initiation temperature of LTE (low temperature exotherm) suggested that all of these tissues described above owe their cold hardiness mechanism mostly to deep supercooling. Deep supercooling in leaf blades was also substantiated with microscopic observations, suggesting that the units of supercooling were minute tissues compartmentalized by longitudinal and cross veins. It was also shown that cooling rates and storage of shoots at -5{degrees}C for 1 to 5 days in the ice-inoculated state did not greatly affect the supercooling ability of leaf blades. Sasa senanensis seemed to exhibit a unique strategy against prolonged subzero temperature, and its leaves would be a good system for the study on mechanisms of deep undercooling in plants.}, added-at = {2019-03-31T01:14:40.000+0100}, author = {Ishikawa, Masaya}, biburl = {https://www.bibsonomy.org/bibtex/2d7eafc857d9bd77eb1c9b1aee1656dd6/dianella}, citeulike-article-id = {1561293}, citeulike-linkout-0 = {http://www.plantphysiol.org/cgi/content/abstract/75/1/196}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16663570}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16663570}, comment = {(private-note)Deep supercooling is this bamboo's mechanism of cold hardiness: The temperature at which they freeze is coincident with LT25 Units of tissue compartmentalised by veins "Extraorgan freezing is characterized by ice segregation from the supercooled organs and their subsequent slow freeze dehydration at naturally occurring cooling rates..." HONG S, E SUCOFF 1980 Units of freezing of deep supercooled water in woody xylem. Plant Physiol 66: 40-45 exotherms measured at the cooling rates of 2, 5, and 37C/h HTE was produced in storage, before DTA. HTE was not related to injury of leaves and might arise from the freezing of water in vascular tissues and/or intracellular space. sclerenchyma, bundle sheath cells, and vascular bundles may work together as a barrier against ice intrusion or to delay ice growth from one unit to another. However, remaining unknown are actual mechanisms to block ice growth from one unit to another or from ice in vascular bundles to mesophyll cells in the units.}, day = 1, interhash = {7aaa6c6e0fe629a911d680591359e185}, intrahash = {d7eafc857d9bd77eb1c9b1aee1656dd6}, journal = {Plant Physiol.}, keywords = {anatomy, cellular, citeulikeExport cryo, damage, freezing, grass, ice, intracellular, nucleation, snowgumpapermaybe}, month = may, number = 1, pages = {196--202}, pmid = {16663570}, posted-at = {2007-08-15 01:38:00}, priority = {0}, timestamp = {2019-03-31T01:16:26.000+0100}, title = {{Deep Supercooling in Most Tissues of Wintering Sasa senanensis and Its Mechanism in Leaf Blade Tissues}}, url = {http://www.plantphysiol.org/cgi/content/abstract/75/1/196}, volume = 75, year = 1984 }