%0 Journal Article %1 claus_genetic_2001 %A Claus, H %A Stoevesandt, J %A Frosch, M %A Vogel, U %D 2001 %J Journal of Bacteriology %K Acid Analysis, Bacterial, Bacteriophages, Chromosome Data, Electrophoresis, Enzymes, Genome, Humans, Hybridization, Mapping, Molecular Neisseria Nucleic Plasmids, Sequence meningitidis, {DNA} {Restriction-Modification} %N 8 %P 2570--2575 %R 10.1128/JB.183.8.2570-2575.2001 %T Genetic isolation of meningococci of the electrophoretic type 37 complex %U http://www.ncbi.nlm.nih.gov/pubmed/11274117 %V 183 %X Neisseria meningitidis (the meningococcus) is a naturally competent bacterial species in which intra- and interspecific horizontal gene transfer is a major source of genetic diversity. In strains of the electrophoretic type 37 (ET-37) complex and of the A4 cluster, we identified genomic DNA coding for a novel restriction-modification system and for the tail of a previously unidentified prophage. Furthermore, a novel 7.2-kb DNA segment restricted to clones of the ET-37 complex and the A4 cluster was isolated and shown to occur both as a plasmid (pJS-B) and as a chromosomal integration. Neither the genomic loci nor pJS-B was present in ET-5 complex, lineage 3, or serogroup A meningococci. The differential distribution of the DNA segments described herein, as well as of opcA, porB, nmeAI, nmeBI, and nmeDI described previously, supports the concept of genetic isolation of hypervirulent lineages responsible for most cases of serogroup C disease worldwide.

@article{claus_genetic_2001, abstract = {Neisseria meningitidis (the meningococcus) is a naturally competent bacterial species in which intra- and interspecific horizontal gene transfer is a major source of genetic diversity. In strains of the electrophoretic type 37 {(ET-37)} complex and of the A4 cluster, we identified genomic {DNA} coding for a novel restriction-modification system and for the tail of a previously unidentified prophage. Furthermore, a novel 7.2-kb {DNA} segment restricted to clones of the {ET-37} complex and the A4 cluster was isolated and shown to occur both as a plasmid {(pJS-B)} and as a chromosomal integration. Neither the genomic loci nor {pJS-B} was present in {ET-5} complex, lineage 3, or serogroup A meningococci. The differential distribution of the {DNA} segments described herein, as well as of {opcA}, {porB}, {nmeAI}, {nmeBI}, and {nmeDI} described previously, supports the concept of genetic isolation of hypervirulent lineages responsible for most cases of serogroup C disease worldwide.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Claus, H and Stoevesandt, J and Frosch, M and Vogel, U}, biburl = {https://www.bibsonomy.org/bibtex/2b20fcbd5945d2639a39e5067301eb04d/ag_vogel}, doi = {10.1128/JB.183.8.2570-2575.2001}, interhash = {803db3db0ce9ba1cdb52a85a1ec555a1}, intrahash = {b20fcbd5945d2639a39e5067301eb04d}, issn = {0021-9193}, journal = {Journal of Bacteriology}, keywords = {Acid Analysis, Bacterial, Bacteriophages, Chromosome Data, Electrophoresis, Enzymes, Genome, Humans, Hybridization, Mapping, Molecular Neisseria Nucleic Plasmids, Sequence meningitidis, {DNA} {Restriction-Modification}}, month = apr, note = {{PMID:} 11274117}, number = 8, pages = {2570--2575}, timestamp = {2011-06-24T11:21:47.000+0200}, title = {Genetic isolation of meningococci of the electrophoretic type 37 complex}, url = {http://www.ncbi.nlm.nih.gov/pubmed/11274117}, volume = 183, year = 2001 }