%0 Journal Article %1 noauthororeditor %A |, Wisam Abdan Wawi AL-Abdullah %D 2023 %J CENTRAL ASIAN JOURNAL OF MEDICAL AND NATURAL SCIENCE %K Cancer, MORC2, expression gene line-1, %N 6 %P 1143-1151 %T Investigation Association between Line-1 And MORC2 Expression in Different Types of Cancers %U https://cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/2202/2240 %V 4 %X Line-1 elements act as an engine in alteration human genomic DNA. These elements continue to insert new insertions facilitating genomic diversity and sometimes causing disease including cancer. Mobilization of line-1 could act as mutagenesis and exon skipping. However, there are different mechanisms can regulate mRNA transcript of line-1 elements.. MORC2 gene was suggested to be a novel participant in regulation line-1 transcription, acting by binding HUSH complex and suppress active line-1 element. Thus, in current project we are interested to evaluate the level expression of line-1 and MORC2 in some cases of cancer compared with healthy group as well as evaluating the correlation between line-1 and MORC2 expression to investigate how regulatory factors affect line-1 expression in several cases of cancer. This study was first assay conducted in Iraq. However, this study started with collecting whole blood samples of patients and healthy group. Then, RNA was extracted and created cDNA to set up RT-qPCR, which used to analyze mRNA level of both genes line-1 and MORC2. Most cases of cancer expressed line-1 including testicular, breast, colon, pharyngeal, stomach, prostate, rectal, pancreatic, brain, lung, and bone respectively at different levels of p-value. Brain cancer was highly expressed line-1. MORC2 gene was expressed in rectal, pancreatic, brain, lymphoma, testicular, cervical, ovarian, pharyngeal, bone, lung, and leukemia at p-value 0.05. Line-1 is substantially more elevated than MORC2 in several cancer types, and Line-1 expression has a significantly negative correlation with MORC2 expression in cancer patients.

@article{noauthororeditor, abstract = {Line-1 elements act as an engine in alteration human genomic DNA. These elements continue to insert new insertions facilitating genomic diversity and sometimes causing disease including cancer. Mobilization of line-1 could act as mutagenesis and exon skipping. However, there are different mechanisms can regulate mRNA transcript of line-1 elements.. MORC2 gene was suggested to be a novel participant in regulation line-1 transcription, acting by binding HUSH complex and suppress active line-1 element. Thus, in current project we are interested to evaluate the level expression of line-1 and MORC2 in some cases of cancer compared with healthy group as well as evaluating the correlation between line-1 and MORC2 expression to investigate how regulatory factors affect line-1 expression in several cases of cancer. This study was first assay conducted in Iraq. However, this study started with collecting whole blood samples of patients and healthy group. Then, RNA was extracted and created cDNA to set up RT-qPCR, which used to analyze mRNA level of both genes line-1 and MORC2. Most cases of cancer expressed line-1 including testicular, breast, colon, pharyngeal, stomach, prostate, rectal, pancreatic, brain, lung, and bone respectively at different levels of p-value. Brain cancer was highly expressed line-1. MORC2 gene was expressed in rectal, pancreatic, brain, lymphoma, testicular, cervical, ovarian, pharyngeal, bone, lung, and leukemia at p-value 0.05. Line-1 is substantially more elevated than MORC2 in several cancer types, and Line-1 expression has a significantly negative correlation with MORC2 expression in cancer patients.}, added-at = {2024-02-17T12:18:00.000+0100}, author = {|, Wisam Abdan Wawi AL-Abdullah}, biburl = {https://www.bibsonomy.org/bibtex/24ee66bad71bd65b45392a9e7d0bdc3ca/centralasian_20}, interhash = {84afda28704ffe2b8e6212d97afca8e9}, intrahash = {4ee66bad71bd65b45392a9e7d0bdc3ca}, issn = {2660-4159}, journal = {CENTRAL ASIAN JOURNAL OF MEDICAL AND NATURAL SCIENCE}, keywords = {Cancer, MORC2, expression gene line-1,}, language = {english}, month = dec, number = 6, pages = {1143-1151}, timestamp = {2024-02-17T12:18:00.000+0100}, title = {Investigation Association between Line-1 And MORC2 Expression in Different Types of Cancers}, url = {https://cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/2202/2240}, volume = 4, year = 2023 }