%0 Journal Article %1 zahran2021local %A Zahran, E. M. %A Abdelmohsen, U. R. %A Shalash, M. M. %A Salem, M. A. %A Khalil, H. E. %A Desoukey, S. Y. %A Fouad, M. A. %A Krischke, M. %A Mueller, M. %A Kamel, M. S. %D 2021 %J Nat Prod Res %K Anesthesia, Local myOwn %N 22 %P 4757-4763 %R 10.1080/14786419.2020.1719489 %T Local anaesthetic potential, metabolic profiling, molecular docking and in silico ADME studies of Ocimum forskolei, family Lamiaceae %U https://www.ncbi.nlm.nih.gov/pubmed/32000524 %V 35 %X The present study aimed to detect the bioactive metabolites from Ocimum forskolei aerial parts which are responsible for the local anaesthetic activity of the ethyl acetate fraction. Following a bioassay-guided fractionation, twelve compounds were dereplicated from the ethyl acetate fraction which was the most potent one with a mean onset of action (1.43 +/- 0.07****) min compared to tetracaine as a positive control (1.37 +/- 0.07****) min. These compounds, along with seven other compounds (isolated by diverse chromatographic techniques) were subjected to a molecular docking study to declare the top scoring compounds predicted to be responsible for such activity. The results highlighted Rabdosiin and Apigenin-7-O-rutinoside as the main bioactive leaders of the local anaesthesia via forming multiple H- bonding with the sodium ion channels leading to their blockade and loss of pain sensation, which strongly supports the use of O. forskolei as a local anaesthetic agent.

@article{zahran2021local, abstract = {The present study aimed to detect the bioactive metabolites from Ocimum forskolei aerial parts which are responsible for the local anaesthetic activity of the ethyl acetate fraction. Following a bioassay-guided fractionation, twelve compounds were dereplicated from the ethyl acetate fraction which was the most potent one with a mean onset of action (1.43 +/- 0.07****) min compared to tetracaine as a positive control (1.37 +/- 0.07****) min. These compounds, along with seven other compounds (isolated by diverse chromatographic techniques) were subjected to a molecular docking study to declare the top scoring compounds predicted to be responsible for such activity. The results highlighted Rabdosiin and Apigenin-7-O-rutinoside as the main bioactive leaders of the local anaesthesia via forming multiple H- bonding with the sodium ion channels leading to their blockade and loss of pain sensation, which strongly supports the use of O. forskolei as a local anaesthetic agent.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Zahran, E. M. and Abdelmohsen, U. R. and Shalash, M. M. and Salem, M. A. and Khalil, H. E. and Desoukey, S. Y. and Fouad, M. A. and Krischke, M. and Mueller, M. and Kamel, M. S.}, biburl = {https://www.bibsonomy.org/bibtex/205887e9cdd4c3b331be7c584b351711e/jvsi_all}, doi = {10.1080/14786419.2020.1719489}, interhash = {87595382646397553a2c01045551195b}, intrahash = {05887e9cdd4c3b331be7c584b351711e}, issn = {1478-6427 (Electronic) 1478-6419 (Linking)}, journal = {Nat Prod Res}, keywords = {Anesthesia, Local myOwn}, note = {Zahran, Eman Maher Abdelmohsen, Usama Ramadan Shalash, Mahmoud M Salem, M Alaraby Khalil, Hany Ezzat Desoukey, Samar Yehia Fouad, Mostafa Ahmed Krischke, Markus Mueller, Martin Kamel, Mohamed Salah eng England 2020/02/01 Nat Prod Res. 2021 Nov;35(22):4757-4763. doi: 10.1080/14786419.2020.1719489. Epub 2020 Jan 30.}, number = 22, pages = {4757-4763}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {Local anaesthetic potential, metabolic profiling, molecular docking and in silico ADME studies of Ocimum forskolei, family Lamiaceae}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/32000524}, volume = 35, year = 2021 }