%0 Journal Article %1 Woods1993 %A Woods, C. R. %A Versalovic, J. %A Koeuth, T. %A Lupski, J. R. %D 1993 %J J Clin Microbiol %K bacteria highthroughput gene-expression %N 7 %P 1927--1931 %T Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. %V 31 %X Repetitive element sequence-based polymerase chain reaction (rep-PCR) enables the generation of DNA fingerprints which discriminate bacterial species and strains. We describe the application of whole-cell methods which allow specimens from broth cultures or colonies from agar plates to be utilized directly in the PCR mixture. The rep-PCR-generated DNA fingerprints obtained with whole-cell samples match results obtained with genomic DNA templates. Examples with different gram-negative bacteria (e.g., Citrobacter diversus, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive bacteria (e.g., Staphylococcus aureus and Streptococcus pneumoniae) are demonstrated. Rapid specimen preparation methods enable rep-PCR-based fingerprinting to be completed in several hours and, therefore, allows the timely analysis of epidemiological relationships.

@article{Woods1993, abstract = {Repetitive element sequence-based polymerase chain reaction (rep-PCR) enables the generation of DNA fingerprints which discriminate bacterial species and strains. We describe the application of whole-cell methods which allow specimens from broth cultures or colonies from agar plates to be utilized directly in the PCR mixture. The rep-PCR-generated DNA fingerprints obtained with whole-cell samples match results obtained with genomic DNA templates. Examples with different gram-negative bacteria (e.g., Citrobacter diversus, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive bacteria (e.g., Staphylococcus aureus and Streptococcus pneumoniae) are demonstrated. Rapid specimen preparation methods enable rep-PCR-based fingerprinting to be completed in several hours and, therefore, allows the timely analysis of epidemiological relationships.}, added-at = {2013-07-23T00:01:46.000+0200}, author = {Woods, C. R. and Versalovic, J. and Koeuth, T. and Lupski, J. R.}, biburl = {https://www.bibsonomy.org/bibtex/2fce327dc98b16e9cf7236847970b1105/aorchid}, description = {seems to be the best paper to cite for rep-PCR on clinical isolates.}, file = {:ID_General/JClinMicrobiol.31.1927.pdf:PDF}, groups = {public}, institution = {Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030.}, interhash = {898ecea0fb5d83d0706212afc481aee4}, intrahash = {fce327dc98b16e9cf7236847970b1105}, journal = {J Clin Microbiol}, keywords = {bacteria highthroughput gene-expression}, language = {eng}, medline-pst = {ppublish}, month = Jul, number = 7, pages = {1927--1931}, pmid = {8349778}, timestamp = {2013-07-23T00:01:46.000+0200}, title = {Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates.}, username = {aorchid}, volume = 31, year = 1993 }