%0 Journal Article %1 Martinez-Garcia2014 %A Martínez-García, Esteban %A Nikel, Pablo I. %A Chavarría, Max %A de Lorenzo, Víctor %D 2014 %J Environmental Microbiology %K bacteria flagella motility pputida pseudomonas %N 1 %P 291--303 %R 10.1111/1462-2920.12309 %T The metabolic cost of flagellar motion in Pseudomonas putida KT2440 %U http://dx.doi.org/10.1111/1462-2920.12309 %V 16 %X Although the flagellar machinery of environmental bacteria endows cells with a phenomenal survival device, it also consumes much of the metabolic currency necessary for fuelling such a vigorous nano-motor. The physiological cost of flagella-related functions of the soil bacterium Pseudomonas putida KT2440 was examined and quantified through the deletion of a ∼ 70 kb DNA segment of the genome (∼ 1.1%), which includes relevant structural and regulatory genes in this micro-organism. The resulting strain lacked the protruding polar cords that define flagella in the wild-type P. putida strain and was unable of any swimming motility while showing a significant change in surface hydrophobicity. However, these deficiencies were otherwise concomitant with clear physiological advantages: rapid adaptation of the deleted strain to both glycolytic and gluconeogenic carbon sources, increased energy charge and, most remarkably, improved tolerance to oxidative stress, reflecting an increased NADPH/NADP+ ratio. These qualities improve the endurance of non-flagellated cells to the metabolic fatigue associated with rapid growth in rich medium. Thus, flagellar motility represents the archetypal tradeoff involved in acquiring environmental advantages at the cost of a considerable metabolic burden.

@article{Martinez-Garcia2014, abstract = {Although the flagellar machinery of environmental bacteria endows cells with a phenomenal survival device, it also consumes much of the metabolic currency necessary for fuelling such a vigorous nano-motor. The physiological cost of flagella-related functions of the soil bacterium Pseudomonas putida KT2440 was examined and quantified through the deletion of a ∼ 70 kb DNA segment of the genome (∼ 1.1%), which includes relevant structural and regulatory genes in this micro-organism. The resulting strain lacked the protruding polar cords that define flagella in the wild-type P. putida strain and was unable of any swimming motility while showing a significant change in surface hydrophobicity. However, these deficiencies were otherwise concomitant with clear physiological advantages: rapid adaptation of the deleted strain to both glycolytic and gluconeogenic carbon sources, increased energy charge and, most remarkably, improved tolerance to oxidative stress, reflecting an increased NADPH/NADP+ ratio. These qualities improve the endurance of non-flagellated cells to the metabolic fatigue associated with rapid growth in rich medium. Thus, flagellar motility represents the archetypal tradeoff involved in acquiring environmental advantages at the cost of a considerable metabolic burden.}, added-at = {2014-12-03T15:30:42.000+0100}, author = {Martínez-García, Esteban and Nikel, Pablo I. and Chavarría, Max and de Lorenzo, Víctor}, biburl = {https://www.bibsonomy.org/bibtex/256ac35961ad69f1e6a292d6592194675/quantentunnel}, description = {The metabolic cost of flagellar motion in Pseudomonas putida KT2440 - Martínez-García - 2013 - Environmental Microbiology - Wiley Online Library}, doi = {10.1111/1462-2920.12309}, interhash = {8a4749a5b972ee434c27636397f95aea}, intrahash = {56ac35961ad69f1e6a292d6592194675}, issn = {1462-2920}, journal = {Environmental Microbiology}, keywords = {bacteria flagella motility pputida pseudomonas}, number = 1, pages = {291--303}, timestamp = {2014-12-03T15:30:42.000+0100}, title = {The metabolic cost of flagellar motion in Pseudomonas putida KT2440}, url = {http://dx.doi.org/10.1111/1462-2920.12309}, volume = 16, year = 2014 }