%0 Journal Article %1 ijbiotech %A Bachtiar, Endang Winiati %A Smooker, Peter %A Coloe, Peter J %D 2009 %I Research Center for Biotechnology %J Indonesian Journal of Biotechnology %K DNA HIV Recombinant, Salmonella, vaccine, %N 2 %P 1188-1195 %T Construction and Immunogenicity Testing of Salmonella, STM1 Vaccine Vector Expressing HIV-1 Antigen %U http://ijbiotech.ugm.ac.id/index.php/biotech/article/view/113 %V 14 %X AbstractObjective of this study: to determine the ability of Salmonella enterica serovar Typhimurium STM1 as a delivery vehicle for the HIV p24 gene and HIV env gene. The STM1 delivery HIV-p24 vaccination was carried out in the form of a recombinant or a DNA vaccine whereas only a DNA vaccine was used for HIV env. Naked DNA vaccination was also tested and immune responses were evaluated following immunisation in mouse model. Results: vaccination cellular immune responses induced by recombinant p24 STM1 (STM1/pHly-p24) were greater than those elicited by the p24 DNA vaccine in STM1 (STM1/VR-p24), (but statistically not significance) than those induced by oral vaccination. However, IgA responses induced by oral vaccination with either a recombinant or DNA vaccine of p24 in STM1 are higher than those induced by IP vaccination. Conclusions: This result confirmsother studies that Salmonella was able to deliver HIV antigens to the immune system and induced specific immune responses to the HIV antigen.

@article{ijbiotech, abstract = {AbstractObjective of this study: to determine the ability of Salmonella enterica serovar Typhimurium STM1 as a delivery vehicle for the HIV p24 gene and HIV env gene. The STM1 delivery HIV-p24 vaccination was carried out in the form of a recombinant or a DNA vaccine whereas only a DNA vaccine was used for HIV env. Naked DNA vaccination was also tested and immune responses were evaluated following immunisation in mouse model. Results: vaccination cellular immune responses induced by recombinant p24 STM1 (STM1/pHly-p24) were greater than those elicited by the p24 DNA vaccine in STM1 (STM1/VR-p24), (but statistically not significance) than those induced by oral vaccination. However, IgA responses induced by oral vaccination with either a recombinant or DNA vaccine of p24 in STM1 are higher than those induced by IP vaccination. Conclusions: This result confirmsother studies that Salmonella was able to deliver HIV antigens to the immune system and induced specific immune responses to the HIV antigen.}, added-at = {2010-12-15T00:27:17.000+0100}, author = {Bachtiar, Endang Winiati and Smooker, Peter and Coloe, Peter J}, biburl = {https://www.bibsonomy.org/bibtex/20c35e5a8079d5ae1770280c34db31b55/ijbiotech}, interhash = {96ca2001ab77d01eddee69a020d72bf7}, intrahash = {0c35e5a8079d5ae1770280c34db31b55}, journal = {Indonesian Journal of Biotechnology}, keywords = {DNA HIV Recombinant, Salmonella, vaccine,}, number = 2, pages = {1188-1195}, publisher = {Research Center for Biotechnology}, timestamp = {2010-12-15T00:27:19.000+0100}, title = {Construction and Immunogenicity Testing of Salmonella, STM1 Vaccine Vector Expressing HIV-1 Antigen}, url = {http://ijbiotech.ugm.ac.id/index.php/biotech/article/view/113}, volume = 14, year = 2009 }