%0 Journal Article %1 frhlich_occurrence_2007 %A Fröhlich, Jürgen %A Koustiane, Christine %A Kämpfer, Peter %A Rosselló-Mora, Ramón %A Valens, Maria %A Berchtold, Manfred %A Kuhnigk, Thomas %A Hertel, Horst %A Maheshwari, Dinesh K %A König, Helmut %D 2007 %J Systematic and applied microbiology %K 16S Animals Bacterial DNA Digestive_System Fatty_Acids IFZ Isoptera Molecular_Sequence_Data Phylogeny Plants RNA Rhizobiaceae Ribosomal Sinorhizobium Soil_Microbiology Symbiosis %P 68-74 %T Occurrence of rhizobia in the gut of the higher termite Nasutitermes nigriceps %V 30 %X Wood-eating termites feed on a diet highly deficient in nitrogen. They must complement their diet with the aid of nitrogen-fixing bacteria. Nitrogen fixation in the gut has been demonstrated, but information about nitrogen-fixing bacteria in pure culture is scarce. From the higher termite Nasutitermes nigriceps the symbiotic bacterial strain M3A was isolated, which thrives in the hindgut contents. The Gram-negative strain exhibited similarities to the species of the genus Ensifer (including Sinorhizobium) on the basis of morphological and physiological/biochemical features. The 16S rRNA gene analysis showed the highest sequence similarity of the isolate M3A to Ensifer adhaerens (\textgreater99\%; ATCC 33499). The DNA-DNA hybridization revealed a similarity of 66\% with E. adhaerens (NCIMB12342(T)). In contrast to the type strain the isolate M3A possesses the capacity to nodulate plant roots. This is the first report on the detailed identification of a rhizobia-related strain from the intestinal tract of animals. Strain M3A has been deposited with two culture collections (DSM10169; ATCC BAA-396).

@article{frhlich_occurrence_2007, abstract = {Wood-eating termites feed on a diet highly deficient in nitrogen. They must complement their diet with the aid of nitrogen-fixing bacteria. Nitrogen fixation in the gut has been demonstrated, but information about nitrogen-fixing bacteria in pure culture is scarce. From the higher termite Nasutitermes nigriceps the symbiotic bacterial strain M3A was isolated, which thrives in the hindgut contents. The Gram-negative strain exhibited similarities to the species of the genus Ensifer (including Sinorhizobium) on the basis of morphological and physiological/biochemical features. The 16S rRNA gene analysis showed the highest sequence similarity of the isolate M3A to Ensifer adhaerens ({\textgreater}99\%; ATCC 33499). The DNA-DNA hybridization revealed a similarity of 66\% with E. adhaerens (NCIMB12342(T)). In contrast to the type strain the isolate M3A possesses the capacity to nodulate plant roots. This is the first report on the detailed identification of a rhizobia-related strain from the intestinal tract of animals. Strain M3A has been deposited with two culture collections (DSM10169; ATCC BAA-396).}, added-at = {2008-05-07T16:02:06.000+0200}, author = {Fröhlich, Jürgen and Koustiane, Christine and Kämpfer, Peter and Rosselló-Mora, Ramón and Valens, Maria and Berchtold, Manfred and Kuhnigk, Thomas and Hertel, Horst and Maheshwari, Dinesh K and König, Helmut}, biburl = {https://www.bibsonomy.org/bibtex/2cd4765ea013d2a753eb4ae4bced9fe3b/recymikrobio}, interhash = {a0b1977d781b726e9150dece2bbaacb3}, intrahash = {cd4765ea013d2a753eb4ae4bced9fe3b}, issn = {07232020}, journal = {Systematic and applied microbiology}, keywords = {16S Animals Bacterial DNA Digestive_System Fatty_Acids IFZ Isoptera Molecular_Sequence_Data Phylogeny Plants RNA Rhizobiaceae Ribosomal Sinorhizobium Soil_Microbiology Symbiosis}, note = {PMID: 16584862}, pages = {68-74}, timestamp = {2008-07-18T13:58:11.000+0200}, title = {Occurrence of rhizobia in the gut of the higher termite Nasutitermes nigriceps}, volume = 30, year = 2007 }