%0 Journal Article %1 forster2019woundinginduced %A Forster, S. %A Schmidt, L. K. %A Kopic, E. %A Anschutz, U. %A Huang, S. %A Schlucking, K. %A Koster, P. %A Waadt, R. %A Larrieu, A. %A Batistic, O. %A Rodriguez, P. L. %A Grill, E. %A Kudla, J. %A Becker, D. %D 2019 %J Dev Cell %K Abscisic Acid/*metabolism myOwn %N 1 %P 87-99 e6 %R 10.1016/j.devcel.2018.11.014 %T Wounding-Induced Stomatal Closure Requires Jasmonate-Mediated Activation of GORK K(+) Channels by a Ca(2+) Sensor-Kinase CBL1-CIPK5 Complex %U https://www.ncbi.nlm.nih.gov/pubmed/30528785 %V 48 %X Guard cells integrate various hormone signals and environmental cues to balance plant gas exchange and transpiration. The wounding-associated hormone jasmonic acid (JA) and the drought hormone abscisic acid (ABA) both trigger stomatal closure. In contrast to ABA however, the molecular mechanisms of JA-induced stomatal closure have remained largely elusive. Here, we identify a fast signaling pathway for JA targeting the K(+) efflux channel GORK. Wounding triggers both local and systemic stomatal closure by activation of the JA signaling cascade followed by GORK phosphorylation and activation through CBL1-CIPK5 Ca(2+) sensor-kinase complexes. GORK activation strictly depends on plasma membrane targeting and Ca(2+) binding of CBL1-CIPK5 complexes. Accordingly, in gork, cbl1, and cipk5 mutants, JA-induced stomatal closure is specifically abolished. The ABA-coreceptor ABI2 counteracts CBL1-CIPK5-dependent GORK activation. Hence, JA-induced Ca(2+) signaling in response to biotic stress converges with the ABA-mediated drought stress pathway to facilitate GORK-mediated stomatal closure upon wounding.

@article{forster2019woundinginduced, abstract = {Guard cells integrate various hormone signals and environmental cues to balance plant gas exchange and transpiration. The wounding-associated hormone jasmonic acid (JA) and the drought hormone abscisic acid (ABA) both trigger stomatal closure. In contrast to ABA however, the molecular mechanisms of JA-induced stomatal closure have remained largely elusive. Here, we identify a fast signaling pathway for JA targeting the K(+) efflux channel GORK. Wounding triggers both local and systemic stomatal closure by activation of the JA signaling cascade followed by GORK phosphorylation and activation through CBL1-CIPK5 Ca(2+) sensor-kinase complexes. GORK activation strictly depends on plasma membrane targeting and Ca(2+) binding of CBL1-CIPK5 complexes. Accordingly, in gork, cbl1, and cipk5 mutants, JA-induced stomatal closure is specifically abolished. The ABA-coreceptor ABI2 counteracts CBL1-CIPK5-dependent GORK activation. Hence, JA-induced Ca(2+) signaling in response to biotic stress converges with the ABA-mediated drought stress pathway to facilitate GORK-mediated stomatal closure upon wounding.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Forster, S. and Schmidt, L. K. and Kopic, E. and Anschutz, U. and Huang, S. and Schlucking, K. and Koster, P. and Waadt, R. and Larrieu, A. and Batistic, O. and Rodriguez, P. L. and Grill, E. and Kudla, J. and Becker, D.}, biburl = {https://www.bibsonomy.org/bibtex/21d27d8e9acc454899925a6a585467b73/jvsi_all}, doi = {10.1016/j.devcel.2018.11.014}, interhash = {a391b69f28ba0dcc1961ac30b115b843}, intrahash = {1d27d8e9acc454899925a6a585467b73}, issn = {1878-1551 (Electronic) 1534-5807 (Linking)}, journal = {Dev Cell}, keywords = {Abscisic Acid/*metabolism myOwn}, note = {Forster, Sabrina Schmidt, Lena K Kopic, Eva Anschutz, Uta Huang, Shouguang Schlucking, Kathrin Koster, Philipp Waadt, Rainer Larrieu, Antoine Batistic, Oliver Rodriguez, Pedro L Grill, Erwin Kudla, Jorg Becker, Dirk eng Research Support, Non-U.S. Gov't 2018/12/12 Dev Cell. 2019 Jan 7;48(1):87-99.e6. doi: 10.1016/j.devcel.2018.11.014. Epub 2018 Dec 6.}, number = 1, pages = {87-99 e6}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {Wounding-Induced Stomatal Closure Requires Jasmonate-Mediated Activation of GORK K(+) Channels by a Ca(2+) Sensor-Kinase CBL1-CIPK5 Complex}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/30528785}, volume = 48, year = 2019 }