%0 Journal Article %1 li2021optimized %A Li, K. %A Prada, J. %A Damineli, D. S. C. %A Liese, A. %A Romeis, T. %A Dandekar, T. %A Feijo, J. A. %A Hedrich, R. %A Konrad, K. R. %D 2021 %J New Phytol %K Abscisic Acid myOwn %N 6 %P 2292-2310 %R 10.1111/nph.17202 %T An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca(2+) and H(+) reveals new insights into ion signaling in plants %U https://www.ncbi.nlm.nih.gov/pubmed/33455006 %V 230 %X Whereas the role of calcium ions (Ca(2+) ) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca(2+) and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca(2+) - and pH-dynamics revealed interconnections previously not described. In tobacco PTs, we demonstrated Ca(2+) -dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca(2+) . In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca(2+) elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca(2+) -increases and cytosolic acidification by c. 2 min, suggesting a Ca(2+) /pH-independent early pathogen signaling step. Imaging Ca(2+) and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca(2+) - and pH-responses. We propose close interrelation in Ca(2+) - and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement.

@article{li2021optimized, abstract = {Whereas the role of calcium ions (Ca(2+) ) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca(2+) and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca(2+) - and pH-dynamics revealed interconnections previously not described. In tobacco PTs, we demonstrated Ca(2+) -dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca(2+) . In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followed by Ca(2+) elevation. Preventing the alkalization blocked GC ABA-responses and even opened stomata in the presence of ABA, disclosing an important pH-dependent GC signaling node. In MCs, a flg22-induced membrane depolarization preceded Ca(2+) -increases and cytosolic acidification by c. 2 min, suggesting a Ca(2+) /pH-independent early pathogen signaling step. Imaging Ca(2+) and pH resolved similar cytosol and nuclear signals and demonstrated flg22, but not ABA and hydrogen peroxide to initiate rapid membrane voltage-, Ca(2+) - and pH-responses. We propose close interrelation in Ca(2+) - and pH-signaling that is cell type- and stimulus-specific and the pH having crucial roles in regulating PT growth and stomata movement.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Li, K. and Prada, J. and Damineli, D. S. C. and Liese, A. and Romeis, T. and Dandekar, T. and Feijo, J. A. and Hedrich, R. and Konrad, K. R.}, biburl = {https://www.bibsonomy.org/bibtex/2d046c2639bd301e748fb5a85d5b57970/jvsi_all}, doi = {10.1111/nph.17202}, interhash = {a4a4ccf503ca3b5f69061b1efbbdfa47}, intrahash = {d046c2639bd301e748fb5a85d5b57970}, issn = {1469-8137 (Electronic) 0028-646X (Print) 0028-646X (Linking)}, journal = {New Phytol}, keywords = {Abscisic Acid myOwn}, note = {Li, Kunkun Prada, Juan Damineli, Daniel S C Liese, Anja Romeis, Tina Dandekar, Thomas Feijo, Jose A Hedrich, Rainer Konrad, Kai Robert eng R01 GM131043/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2021/01/18 New Phytol. 2021 Jun;230(6):2292-2310. doi: 10.1111/nph.17202. Epub 2021 Feb 18.}, number = 6, pages = {2292-2310}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca(2+) and H(+) reveals new insights into ion signaling in plants}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/33455006}, volume = 230, year = 2021 }