%0 Journal Article %1 Bohm1994 %A Bohm, M. %A Lohse, M. J. %D 1994 %J Eur Heart J %K AMP-Dependent Animals Chain Cyclic Down-Regulation/genetics Enzymologic/physiology Expression Failure/*genetics Gene Genetic Heart Humans Kinases Kinases/*genetics Messenger/genetics Myocardium/enzymology Norepinephrine/physiology Polymerase Protein RNA, Reaction/methods Receptor Regulation, Transcription, beta-Adrenergic beta/*genetics Adrenergic %P 30-4 %T Quantification of beta-adrenoceptors and beta-adrenoceptor kinase on protein and mRNA levels in heart failure %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7713110 %V 15 Suppl D %X The alterations of the beta-adrenoceptor adenylyl cyclase are reviewed. In failing myocardium, the down-regulation of beta 1-adrenoceptors is accompanied by a decrease in steady state mRNA levels, as studied with quantitative polymerase chain reactions in dilated and ischaemic cardiomyopathy. The density of beta 2-adrenoceptors and beta 2-adrenoceptor mRNA was unchanged in both pathological conditions compared to non-failing myocardium. In addition to down-regulation of beta 1-receptor protein and mRNA, an increased activity of the beta-adrenoceptor kinase (beta-ARK) was observed. Correspondingly, quantification of beta-ARK mRNA by a 5' and a middle portion PCR-product suggests that increased enzyme activity could be due to increased transcription. In summary, decreased steady state levels of beta 1-adrenoceptor mRNA could contribute to reduced beta-adrenoceptor density in failing myocardium. The known uncoupling of beta-adrenoceptors could be due to an increased mRNA expression and activity of beta-ARK.

@article{Bohm1994, abstract = {The alterations of the beta-adrenoceptor adenylyl cyclase are reviewed. In failing myocardium, the down-regulation of beta 1-adrenoceptors is accompanied by a decrease in steady state mRNA levels, as studied with quantitative polymerase chain reactions in dilated and ischaemic cardiomyopathy. The density of beta 2-adrenoceptors and beta 2-adrenoceptor mRNA was unchanged in both pathological conditions compared to non-failing myocardium. In addition to down-regulation of beta 1-receptor protein and mRNA, an increased activity of the beta-adrenoceptor kinase (beta-ARK) was observed. Correspondingly, quantification of beta-ARK mRNA by a 5' and a middle portion PCR-product suggests that increased enzyme activity could be due to increased transcription. In summary, decreased steady state levels of beta 1-adrenoceptor mRNA could contribute to reduced beta-adrenoceptor density in failing myocardium. The known uncoupling of beta-adrenoceptors could be due to an increased mRNA expression and activity of beta-ARK.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Bohm, M. and Lohse, M. J.}, biburl = {https://www.bibsonomy.org/bibtex/2d2229055d7ebe756219f4c9224da6588/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {abf1fe3f809b4919bb317535f3dad0e1}, intrahash = {d2229055d7ebe756219f4c9224da6588}, issn = {0195-668X (Print) 0195-668X (Linking)}, journal = {Eur Heart J}, keywords = {AMP-Dependent Animals Chain Cyclic Down-Regulation/genetics Enzymologic/physiology Expression Failure/*genetics Gene Genetic Heart Humans Kinases Kinases/*genetics Messenger/genetics Myocardium/enzymology Norepinephrine/physiology Polymerase Protein RNA, Reaction/methods Receptor Regulation, Transcription, beta-Adrenergic beta/*genetics Adrenergic}, month = Dec, note = {Bohm, M Lohse, M J Research Support, Non-U.S. Gov't Review England European heart journal Eur Heart J. 1994 Dec;15 Suppl D:30-4.}, pages = {30-4}, shorttitle = {Quantification of beta-adrenoceptors and beta-adrenoceptor kinase on protein and mRNA levels in heart failure}, timestamp = {2010-12-14T18:22:38.000+0100}, title = {Quantification of beta-adrenoceptors and beta-adrenoceptor kinase on protein and mRNA levels in heart failure}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7713110}, volume = {15 Suppl D}, year = 1994 }