%0 Journal Article %1 Nakajima2000220 %A Nakajima, S. %A Nobusawa, E. %A Nakajima, K. %D 2000 %J Virology %K antigenic influenza %N 1 %P 220 - 231 %R DOI: 10.1006/viro.2000.0453 %T Variation in Response among Individuals to Antigenic Sites on the HA Protein of Human Influenza Virus May Be Responsible for the Emergence of Drift Strains in the Human Population %U http://www.sciencedirect.com/science/article/B6WXR-45BCFH5-81/2/34c7a8541a69c191e35e44272b7fd7b9 %V 274 %X Eight convalescent human sera obtained from patients aged 3 to 14 years old, who were infected with influenza A(H3N2) virus during the 1990/1991 influenza season, were characterized by a binding assay with chimeric hemagglutinin (HA) proteins between influenza virus A/Aichi/2/68 and A/Kamata/14/91(H3N2) strains. These sera did not recognize the HA protein of the A/Aichi/2/68 strain but recognized that of the A/Kamata/14/91 strain. The binding assay revealed that these sera recognized only the HA1 domain of A/Kamata/14/91 HA protein. A further assay of the binding of these sera to the chimeric proteins of the HA1 domain revealed that three sera (A-1, A-2, and A-3) from very young patients bound only to region 150-170 (site B1) and one serum (Y-1) bound to regions 96-150 (site A) and 96-170 (sites A and B1). These four sera showed reduced hemagglutination inhibition (HI) activity with the 203v2 strain, a monoclonal variant of the A/Kamata/14/91 strain with two amino acid changes in the HA protein at antigenic sites A and B1. The other four sera (Y-2, G-1, G-2, and A-4) bound to regions 1-96 (site C/E), 96-150 (site A), 96-170 (sites A and B1), and 170-200 (site B2), two of which further bound to region 240-306 (site C); these sera were all fully reactive with the 203v2 strain. All eight sera showed reduced HI reactivity to a drift strain A/Aichi/4/93. Amino acid changes of the A/Aichi/4/93 strain from the A/Kamata/14/91 strain were located at antigenic sites A, B1, B2, and C. We propose a possible model for the emergence of a drift strain A/Aichi/4/93 from an A/Kamata/14/91-like strain by sequential changes during reinfections of individuals starting from A-1-like, next to Y-1-like, and then to Y-2-like populations.

@article{Nakajima2000220, abstract = { Eight convalescent human sera obtained from patients aged 3 to 14 years old, who were infected with influenza A(H3N2) virus during the 1990/1991 influenza season, were characterized by a binding assay with chimeric hemagglutinin (HA) proteins between influenza virus A/Aichi/2/68 and A/Kamata/14/91(H3N2) strains. These sera did not recognize the HA protein of the A/Aichi/2/68 strain but recognized that of the A/Kamata/14/91 strain. The binding assay revealed that these sera recognized only the HA1 domain of A/Kamata/14/91 HA protein. A further assay of the binding of these sera to the chimeric proteins of the HA1 domain revealed that three sera (A-1, A-2, and A-3) from very young patients bound only to region 150-170 (site B1) and one serum (Y-1) bound to regions 96-150 (site A) and 96-170 (sites A and B1). These four sera showed reduced hemagglutination inhibition (HI) activity with the 203v2 strain, a monoclonal variant of the A/Kamata/14/91 strain with two amino acid changes in the HA protein at antigenic sites A and B1. The other four sera (Y-2, G-1, G-2, and A-4) bound to regions 1-96 (site C/E), 96-150 (site A), 96-170 (sites A and B1), and 170-200 (site B2), two of which further bound to region 240-306 (site C); these sera were all fully reactive with the 203v2 strain. All eight sera showed reduced HI reactivity to a drift strain A/Aichi/4/93. Amino acid changes of the A/Aichi/4/93 strain from the A/Kamata/14/91 strain were located at antigenic sites A, B1, B2, and C. We propose a possible model for the emergence of a drift strain A/Aichi/4/93 from an A/Kamata/14/91-like strain by sequential changes during reinfections of individuals starting from A-1-like, next to Y-1-like, and then to Y-2-like populations.}, added-at = {2009-08-04T18:06:17.000+0200}, author = {Nakajima, S. and Nobusawa, E. and Nakajima, K.}, biburl = {https://www.bibsonomy.org/bibtex/2b7c3a70b5743c1d1a38c44e80d890792/ebo}, doi = {DOI: 10.1006/viro.2000.0453}, interhash = {b103086cae0dc73ff7de2912b889882b}, intrahash = {b7c3a70b5743c1d1a38c44e80d890792}, issn = {0042-6822}, journal = {Virology}, keywords = {antigenic influenza}, number = 1, pages = {220 - 231}, timestamp = {2009-08-04T18:07:46.000+0200}, title = {Variation in Response among Individuals to Antigenic Sites on the HA Protein of Human Influenza Virus May Be Responsible for the Emergence of Drift Strains in the Human Population}, url = {http://www.sciencedirect.com/science/article/B6WXR-45BCFH5-81/2/34c7a8541a69c191e35e44272b7fd7b9}, volume = 274, year = 2000 }