%0 Journal Article %1 rigoldi2018review %A Rigoldi, Federica %A Donini, Stefano %A Redaelli, Alberto %A Parisini, Emilio %A Gautieri, Alfonso %B APL Bioengineering %D 2018 %I American Institute of Physics %J APL Bioengineering %K %N 1 %P 011501-- %R 10.1063/1.4997367 %T Review: Engineering of thermostable enzymes for industrial applications %U https://doi.org/10.1063/1.4997367 %V 2 %X The catalytic properties of some selected enzymes have long been exploited to carry out efficient and cost-effective bioconversions in a multitude of research and industrial sectors, such as food, health, cosmetics, agriculture, chemistry, energy, and others. Nonetheless, for several applications, naturally occurring enzymes are not considered to be viable options owing to their limited stability in the required working conditions. Over the years, the quest for novel enzymes with actual potential for biotechnological applications has involved various complementary approaches such as mining enzyme variants from organisms living in extreme conditions (extremophiles), mimicking evolution in the laboratory to develop more stable enzyme variants, and more recently, using rational, computer-assisted enzyme engineering strategies. In this review, we provide an overview of the most relevant enzymes that are used for industrial applications and we discuss the strategies that are adopted to enhance enzyme stability...

@article{rigoldi2018review, abstract = {The catalytic properties of some selected enzymes have long been exploited to carry out efficient and cost-effective bioconversions in a multitude of research and industrial sectors, such as food, health, cosmetics, agriculture, chemistry, energy, and others. Nonetheless, for several applications, naturally occurring enzymes are not considered to be viable options owing to their limited stability in the required working conditions. Over the years, the quest for novel enzymes with actual potential for biotechnological applications has involved various complementary approaches such as mining enzyme variants from organisms living in extreme conditions (extremophiles), mimicking evolution in the laboratory to develop more stable enzyme variants, and more recently, using rational, computer-assisted enzyme engineering strategies. In this review, we provide an overview of the most relevant enzymes that are used for industrial applications and we discuss the strategies that are adopted to enhance enzyme stability...}, added-at = {2018-09-26T12:15:49.000+0200}, author = {Rigoldi, Federica and Donini, Stefano and Redaelli, Alberto and Parisini, Emilio and Gautieri, Alfonso}, biburl = {https://www.bibsonomy.org/bibtex/2b2d7688b0edd3af36d56c26c1ebc740a/magratheaner}, booktitle = {APL Bioengineering}, comment = {doi: 10.1063/1.4997367}, doi = {10.1063/1.4997367}, interhash = {b5c537f2f5cef1080b5a3231175f4457}, intrahash = {b2d7688b0edd3af36d56c26c1ebc740a}, journal = {APL Bioengineering}, keywords = {}, month = jan, number = 1, pages = {011501--}, publisher = {American Institute of Physics}, timestamp = {2018-09-26T12:15:49.000+0200}, title = {Review: Engineering of thermostable enzymes for industrial applications}, url = {https://doi.org/10.1063/1.4997367}, volume = 2, year = 2018 }