%0 Journal Article %1 citeulike:504090 %A Michán, C. %A Delgado, A. %A Haïdour, A. %A Lucchesi, G. %A Ramos, J. L. %C Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Department of Biochemistry and Molecular and Cellular Biology of Plants, Granada, Spain. bb2midoc@lucano.uco.es %D 1997 %J J Bacteriol %K hydroxylamine bio-degradation aromatic-nitro %N 9 %P 3036--3038 %T In vivo construction of a hybrid pathway for metabolism of 4-nitrotoluene in Pseudomonas fluorescens. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9139924 %V 179 %X Pseudomonas fluorescens 410PR grows on 4-nitrobenzoate but does not metabolize 4-nitrotoluene. The TOL pWW0 delta pm plasmid converts 4-nitrotoluene into 4-nitrobenzoate through its upper pathway, but it does not metabolize 4-nitrobenzoate. P. fluorescens 410PR(pWW0 delta pm) transconjugants were isolated and found to be able to grow on 4-nitrotoluene. This phenotype was stable after growth for at least 300 generations without any selective pressure. P. fluorescens 410PR(pWW0 delta pm) converted 4-nitrotoluene into 4-nitrobenzoate via 4-nitrobenzylalcohol and 4-nitrobenzaldehyde. 4-Nitrobenzoate was metabolized via 4-hydroxylaminobenzoate and finally yielded NH4+ and 3,4-dihydroxybenzoate, which was mineralized.

@article{citeulike:504090, abstract = {Pseudomonas fluorescens 410PR grows on 4-nitrobenzoate but does not metabolize 4-nitrotoluene. The TOL pWW0 delta pm plasmid converts 4-nitrotoluene into 4-nitrobenzoate through its upper pathway, but it does not metabolize 4-nitrobenzoate. P. fluorescens 410PR(pWW0 delta pm) transconjugants were isolated and found to be able to grow on 4-nitrotoluene. This phenotype was stable after growth for at least 300 generations without any selective pressure. P. fluorescens 410PR(pWW0 delta pm) converted 4-nitrotoluene into 4-nitrobenzoate via 4-nitrobenzylalcohol and 4-nitrobenzaldehyde. 4-Nitrobenzoate was metabolized via 4-hydroxylaminobenzoate and finally yielded NH4+ and 3,4-dihydroxybenzoate, which was mineralized.}, added-at = {2007-02-02T11:55:53.000+0100}, address = {Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Department of Biochemistry and Molecular and Cellular Biology of Plants, Granada, Spain. bb2midoc@lucano.uco.es}, author = {Michán, C. and Delgado, A. and Haïdour, A. and Lucchesi, G. and Ramos, J. L.}, biburl = {https://www.bibsonomy.org/bibtex/2f83f147fb891be6717a02a14a234e7cf/robert}, citeulike-article-id = {504090}, interhash = {ba009973f48359686370fc07c7485b27}, intrahash = {f83f147fb891be6717a02a14a234e7cf}, issn = {0021-9193}, journal = {J Bacteriol}, keywords = {hydroxylamine bio-degradation aromatic-nitro}, month = May, number = 9, pages = {3036--3038}, priority = {2}, timestamp = {2007-02-02T11:55:53.000+0100}, title = {In vivo construction of a hybrid pathway for metabolism of 4-nitrotoluene in Pseudomonas fluorescens.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9139924}, volume = 179, year = 1997 }