%0 Journal Article %1 Klotz1986 %A Klotz, K. N. %A Lohse, M. J. %D 1986 %J Biochem Biophys Res Commun %K Acids/analysis Affinity Animals Cricetinae Glycoproteins/*analysis Labels Molecular Neuraminic Neuraminidase/pharmacology Purinergic/*analysis Rats Weight Receptor %N 1 %P 406-13 %T The glycoprotein nature of A1 adenosine receptors %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3778456 %V 140 %X A1 adenosine receptors from different tissues and species were photoaffinity labelled and then the carbohydrate content was examined by both enzymatic and chemical treatment. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the labelled membrane receptors shows that neuraminidase treatment alters the electrophoretic mobility of the receptor band indicating the presence of terminal neuraminic acids. Neuraminidase digestion does not influence the binding characteristics of the receptor. The totally deglycosylated receptor protein obtained by chemical treatment has an apparent molecular weight of 32,000.

@article{Klotz1986, abstract = {A1 adenosine receptors from different tissues and species were photoaffinity labelled and then the carbohydrate content was examined by both enzymatic and chemical treatment. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the labelled membrane receptors shows that neuraminidase treatment alters the electrophoretic mobility of the receptor band indicating the presence of terminal neuraminic acids. Neuraminidase digestion does not influence the binding characteristics of the receptor. The totally deglycosylated receptor protein obtained by chemical treatment has an apparent molecular weight of 32,000.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Klotz, K. N. and Lohse, M. J.}, biburl = {https://www.bibsonomy.org/bibtex/28da3ce5219dae86a0356110405c33b79/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {bbc11677f19ecbf7a4c7c23333bcb265}, intrahash = {8da3ce5219dae86a0356110405c33b79}, issn = {0006-291X (Print) 0006-291X (Linking)}, journal = {Biochem Biophys Res Commun}, keywords = {Acids/analysis Affinity Animals Cricetinae Glycoproteins/*analysis Labels Molecular Neuraminic Neuraminidase/pharmacology Purinergic/*analysis Rats Weight Receptor}, month = {Oct 15}, note = {Klotz, K N Lohse, M J Research Support, Non-U.S. Gov't United states Biochemical and biophysical research communications Biochem Biophys Res Commun. 1986 Oct 15;140(1):406-13.}, number = 1, pages = {406-13}, shorttitle = {The glycoprotein nature of A1 adenosine receptors}, timestamp = {2010-12-14T18:20:05.000+0100}, title = {The glycoprotein nature of A1 adenosine receptors}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3778456}, volume = 140, year = 1986 }